LC-MS考察α-香附酮在Caco-2细胞模型中转运特性

Transport Characteristics of α-Cyperone in Caco-2 Cell Model by LC-MS

摘要:

目的：考察α-香附酮在Caco-2细胞模型中转运机制。方法：建立Caco-2细胞模型，考察体积分数和时间对α-香附酮双向转运过程的影响。采用LC-MS测定α-香附酮含量，流动相甲醇（A）-水（B）梯度洗脱（0~5 min，30%~70%A；5~10 min，70%~100%A；15~20 min，30%A），流速0.25 mL·min^-1，计算不同条件下表观渗透系数（Papp）。结果：α-香附酮在2 h内转运量与时间、体积分数呈正相关。不同体积分数α-香附酮从BL侧到AP侧的Papp与AP侧到BL侧的Papp比值均在0.5~1.5。结论：α-香附酮在Caco-2细胞模型中转运特性为被动扩散转运方式。

Abstract:

Objective: To investigate transport mechanisms of α-cyperone in Caco-2 cell model by LC-MS. Method: Caco-2 cell model was established,effects of volume fraction and time on bidirectional transport process of α-cyperone were investigated.LC-MS was adopted to determine the content of α-cyperone with mobile phase of methanol(A)-water(B)(0-5 min,30%-70%A;5-10 min,70%-100%A;15-20 min,30%A) and a flow rate of 0.25 mL·min^-1,values of apparent permeability coefficient(Papp) under different conditions were calculated. Result: Both transport amounts of AP-to-BL and BL-to-AP increased with increasing of volume fraction and time within 2 h.Papp kept 0.5-1.5 at different volume fractions of α-cyperone. Conclusion: α-Cyperone could be transported through intestinal mucosa via a passive diffusion mechanism.