HPLC测定决明子中3种萘骈吡喃酮苷含量

Determination of Three Naphthopyrone Glycosides in Cassia obtusifolia by HPLC

摘要:

目的：建立HPLC同时测定决明子中红镰霉素龙胆二糖苷、决明子苷、决明子苷C含量的方法。方法：采用HPLC，Dionex Acclaim C₁₈柱（4.6 mm×250 mm，5 μm），流动相乙腈-四氢呋喃-1%冰醋酸（17：2：81），流速1 mL·min^-1，柱温30 ℃，检测波长278 nm。结果：红镰霉素龙胆二糖苷、决明子苷、决明子苷C的进样量分别在0.218~1.090，0.188~0.940，0.200~1.000 μg和峰面积呈良好的线性关系，回归方程分别为Y=54.017X-0.344 6 （r=0.999 9），Y=78.63X-0.354（r=0.999 9），Y=74.098X-0.433 5 （r=0.999 9）；平均加样回收率分别为98.43%（RSD 1.75%），101.89%（RSD 1.10%），102.46%（RSD 1.15%）。结论：该方法快速、灵敏、准确、可靠、重复性好，可用于中药决明子药材的质量控制。

Abstract:

Objective: To establish a high performance liquid chromatographic method (HPLC) for simultaneous determination of rubrofusarin gentiobioside, casside, cassiaside C in Cassia obtusifolia. Method: The HPLC with Dionex Acclaim C₁₈(4.6 mm×250 mm, 5 μm) column was used, acetonitrile-THF-1% acetic acid (17 ：2 ：81) was used as a mobile phase, with flow rate of 1 mL·min^-1, column temperature at 30 ℃ and detection wavelength at 278 nm. Result: Rubrofusarin gentiobioside, casside, cassiaside C showed good linearity (r=0.999 9) in the range of 0.218-1.09, 0.188-0.94, 0.200-1.000 μg, regressive equation were Y=54.017X-0.344 6 (r=0.999 9),Y=78.63X-0.354(r=0.999 9),Y=74.098X-0.433 5 (r=0.999 9)respectively;the average recoveries of the method were 98.43%, 101.89%, 102.46%, RSD were 1.75%, 1.10%, 1.15%. Conclusion: The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.