HPLC测定不同产地滇牡丹中没食子酸和丹皮酚含量

Content Determination of Trihydroxybenzoic Acid and Paeonol in Paeonia delavayi from Different Producing Area by HPLC

摘要:

目的：建立滇牡丹中没食子酸和丹皮酚含量测定方法，并比较滇牡丹不同产地及其不同部位中没食子酸和丹皮酚的含量。方法：采用ZORBOX SB-C₁₈（4.6 mm×250 mm，5 μm）色谱柱，以乙腈-0.1%甲酸溶液为流动相，梯度洗脱，流速1.0 mL·min^-1，检测波长275 nm，柱温30 ℃。结果：没食子酸和丹皮酚在0.204 0~2.040 μg和0.204 0~2.040 μg呈良好的线性关系，平均加样回收率（n=6）为101.81%，97.80%。结论：该方法可用于滇牡丹药材的质量控制；不同产地滇牡丹中没食子酸和丹皮酚的含量差异较大。

Abstract:

Objective: To establish determined method of the content of trihydroxybenzoic acid and paeonol in Paeonia delavayi, and compare the content in samples from different areas and samples from different parts. Method: The samples were separated by a ZORBOX SB-C₁₈ (4.6 mm×250 mm, 5 μm) using acetonitrile-0.1% methane acid with water gradient system as mobile phase. The flow rate was 1.0 mL·min^-1, and the detection wavelength for fingerprinting was 275 nm with 30 ℃ for column temperature. Result: There were well linear relationships for trihydroxybenzoic acid and paeonol in range of 0.204 0-2.040 μg and 0.204 0-2.040 μg. And average recovery rates for them (n=6) were 101.81% and 97.80%. Conclusion: The method could be used for quality control to medicinal materials of P. delavayi. Contents of trihydroxybenzoic acid and paeonol were significantly different for medicinal materials collected from different areas.