HPLC同时测定黄连阿胶汤合煎液中芍药苷黄芩苷小檗碱的含量

Simultaneous Determination of Paeoniflorin,Baicalin and Berberine in the Mixed Decoction of Huanglian Ejiaotang by HPLC

摘要:

目的：建立高效液相色谱法同时测定黄连阿胶汤中芍药苷、黄芩苷、小檗碱3种成分的含量。方法：采用Agilent TC-C₁₈色谱柱（4.6 mm×250 mm），流动相乙腈-0.1%磷酸溶液梯度洗脱，流速1.0 mL·min^-1，检测波长240 nm，柱温25 ℃。结果：黄连阿胶汤中芍药苷、黄芩苷、小檗碱平均质量分数分别为1.64%，7.51%，3.30%。结论：该法操作简便、准确且精密度和重复性良好，可用于黄连阿胶汤的质量控制。

Abstract:

Objective: A simple and rapid HPLC method was established for simultaneously determining of paeoniflorin,baicalin and berberine in the mixed decoction of Huanglian Ejiaotang. Method: Agilent TC-C₁₈(4.6 mm×250 mm)chromatographic column,acetonitrile-0.1% phosphoric acid solution gradient elution were applied. Analyses were performed at 35 ℃ with a flowrate of 1.0 mL·min^-1 and UV detection at 240 nm, Result: The content of paeoniflorin,baicalin and berberine were 1.64%,7.51%,3.30% respectively. Conclusion: The developed method can be applied to the intrinsic quality control of Huanglian Ejiaotang.