鹿茸草药材的HPLC指纹图谱

HPLC Fingerprint of Monochasma sheareri

摘要:

目的：建立鹿茸草的HPLC指纹图谱，为鹿茸草的质量控制提供依据。方法：采用HPLC-UV分析鹿茸草的指纹图谱，色谱条件为Agilent HC C₁₈色谱柱（4.6 mm×250 mm，5 μm），以乙腈（A）-0.4%磷酸水（B）梯度洗脱，流速 0.8 mL·min^-1，柱温35 ℃，检测波长254 nm，进样量10 μL。利用中药色谱指纹图谱相似度评价系统（2004A 版）对不同批次鹿茸草的HPLC图进行比较分析。结果：建立了鹿茸草的 HPLC 指纹图谱，以木犀草素为参照峰，确立了鹿茸草指纹图谱中的13个共有峰。结论：所建立的指纹图谱稳定性和重复性均好，可用于鹿茸草药材的常规质量控制。

Abstract:

Objective: To establish the chromatographic fingerprint for Monochasma sheareri. by RP-HPLC. Method: The HPLC fingerprint of M. sheareri were determined on an Agilent HC C₁₈ column (4.6 mm×250 mm,5 μm) eluted with the mobile phase consisted of acetonitrile and 0.4% phosphoric acid in gradient mode, flow rate 0.8 mL·min^-1,column temperature 35 ℃and the detection wavelength was set at 254 nm.The chromatograms of different batches of M. sheareri. were compared by the software of Similarity Evaluation System for Chromatographic Fingerprint of TCM(Version 2004A). Result: HPLC Fingerprint of M. sheareri. was established with luteolin as the reference compound. 13 common peaks were selected as the fingerprint peaks of M. sheareri. Conclusion: The established HPLC fingerprint of M. sheareri. has desirable precision,reproducibility,and can be applied to routine quality control of M. sheareri.