近红外光谱法快速测定枇杷叶中齐墩果酸的含量

Rapid Determination of Oleanolic Acid Content in Eriobotrya japonica by Near-Infrared Spectroscopy

摘要:

目的：建立枇杷叶药材中齐墩果酸含量的近红外光谱定量模型，快速测定枇杷叶中齐墩果酸含量。方法：用高效液相色谱法测定140批枇杷叶中齐墩果酸含量，采用标准归一化法（SNV）、一阶导数法预处理近红外光谱，结合偏最小二乘法建立枇杷叶中齐墩果酸含量的定量模型，并进行了内部交叉验证和18个枇杷叶样品的外部预测验证。结果：所建立的齐墩果酸近红外光谱定量分析模型，内部交叉验证决定系数达到0.939 23，校正均方差为0.106，预测均方差为0.131，内部交叉验证均方差为0.216 75。结论：该方法快速、准确，为枇杷叶中化学成分快速定量和质量控制提供了方法和依据。

Abstract:

Objective: To develop a method for the determination of oleanolic acid content in Eriobotrya japonica by near-infrared spectroscopy. Method: The oleanolic acid content of 140 samples was determined by the method of HPLC. NIR spectrograms were measured, the standard normal variate (SNV) and first derivative were used as spectral preprocessing options, and calibration model of the oleanolic acid content was established by the partial least squares regression analysis. The models were verified by internal cross validation and external predictive validation with 18 loquat samples. Result: The correlation coefficients, the root-mean-square error of calibration, the root-mean-square error of prediction and the root-mean-square error of cross-validation of the calibration model for ethanol extracts content were 0.939 23, 0.106, 0.131 and 0.216 75. Conclusion: The results show that the method is fast, accurate, and can be used as a rapid qualitative of chemical composition and quality control methods of E. japonica.