朱桔叶挥发油化学成分及抑菌活性研究

Chemical Composition of Olatile Oil from Leaf of Citrus erythrosa and Study on Antibacterial Activity

摘要:

目的：研究朱桔叶挥发油化学成分，初步研究其抑菌活性。方法：采用水蒸气蒸馏法提取朱桔叶中的挥发油，气相色谱-质谱联用技术（GC-MS）和峰面积归一化法分析其化学成分和相对含量；采用微量二倍连续梯度稀释法确定挥发油杀菌效果。结果：通过GC-MS从朱桔叶挥发油中分离41种化合物，鉴定36种。柑橘叶挥发油的主要成分为：r-榄香烯（30.691%）、β-石竹烯（18.895%）、水芹烯（10.814%）、2-异丙基-甲苯（7.977%）、α-石竹烯（5.260%）、α-金合欢烯（4.805%）等；初步的抑菌试验结果显示朱桔叶挥发油对大肠埃希菌的抑制效果[最小抑菌浓度（MIC）3.9 g·L^-1，最小杀菌浓度（MBC）5.6 g·L^-1]最为显著，其次依次为金黄色葡萄球菌、枯草芽孢杆菌和假丝酵母。次为金黄色葡萄球菌、枯草芽孢杆菌和假丝酵母。结论：通过GC-MS测定和鉴别了朱桔叶挥发油化学成分，抗菌实验表明其挥发油具有一定的抗菌活性，为朱桔叶挥发油的开发利用提供实验依据。

Abstract:

Objective: Steam distillation extraction followed by gas chromatography-mass spectrometry (GC-MS) combined with quantification by peak area normalization method was used to analyze volatile compounds from leaf of Citrus erythrosa Tanaka. Method: Analysised bactericidal concentration of olatile compounds with the agar dilution method. Result: Showed that 36 compounds were identified with gc-ms, which the predominant compounds were r-elemene (30.691%), β-caryophyllene (18.895%), phellandrene (10.814%), benzene-1-methyl-2-(1-meth-ylethy) (7.977%), α-caryophyllene (5.260%) and α-farnesene (4.805%) et al. Results of experiments on antimicrobial activities showed that volatile component had antibacterial effects. Results Bacteriostatic test in vitro express that olatile oil to escherichia coli inhibitory effect in the most significant(MIC 3.9 g·L^-1,MBC 5.6 g·L^-1), and followed by staphylococcus aureus, bacillus subtilis and candida. Conclusion: GC-MS could be used to identify and determine content of volatile components of leaf of C. erythrosa,and have a little of antimicrobial activity. This study could provide experimental basis for development and utilization of leaf of C. erythrosa.