神香草与大苞荆芥体外抗氧化活性研究

Antioxidative Activity of Hyssopus cuspidatus and Nepeta bracteata

摘要:

目的：研究神香草与大苞荆芥乙醇提取物不同极性部位的体外抗氧化活性。方法：两种药材乙醇提取物依次用石油醚、二氯甲烷、乙酸乙酯和正丁醇萃取。采用DPPH·自由基法、邻苯三酚自氧化法、铁离子还原法、羟基自由基法等方法，以维生素C（Vc）为参照，分别研究两种药材不同萃取物的抗氧化活性。并计算各组分的IC₅₀。结果：神香草和大苞荆芥乙醇提取物的不同萃取部分具有不同程度的清除自由基能力和还原能力，其中用DPPH法测得神香草各萃取部分半数抑制率（IC₅₀）分别为：HCA（0.201 7），HCP（0.930 6），HCC（0.918 5），HCE（0.028 2），HCB（0.097 4），HCW（0.255 0）g·L^-1；大苞荆芥各萃取部分IC₅₀分别为：HbA（0.147 6），NbP（0.124 9），Nb（0.331 1），NbE（0.047 2），NbB（0.089 4），NbW（0.190 8）g·L^-1。结论：神香草与大苞荆芥均具有一定抗氧化活性，为进一步分离抗氧化的成分提供了实验依据。

Abstract:

Objective: Antioxidative activities of different polarity parts in extracts with ethanol from Hyssopus cuspidatus and Nepeta bracteata were studied in vitro. Method: In vitro chemical simulation conditions, the antioxidant properties of different parts in ethanol extracts of H. cuspidatus and N. bracteata were evaluated by different antioxidant tests, including 1, 1 diphenyl-2-bitter hydrazo (DPPH·) and (O₂^-·) free radical scavenging ability, pyrogallol autoxidation and iron ion reduction. Result: The different polarity parts in extracts with ethanol from the two medicinal materials all have free radical scavenging and reduction abilities to some extent. Conclusion: H. cuspidatus and N. bracteata have antioxidant activities, which will supply the separating base of antioxidative components from them in further research.