清伙灵对大鼠积滞化热证干预作用的代谢组学研究

Metabonomic Study of Intervention Effects of Qinghuoling on Stagnant Heat Syndrome in Rats

摘要:

目的：运用代谢组学方法研究积滞化热模型大鼠机体代谢表型的变化及清伙灵发挥干预作用的相关代谢途径。方法：实验分正常组、模型组和清伙灵组。模型组和清伙灵组给予特制饲料并ip阿托品（0.25 mg·kg^-1），每日2次，连续造模7 d，从造模第4天开始，ig清伙灵（2.64 g·kg^-1），每日1次。大鼠血清和尿液的代谢指纹利用核磁共振氢谱（¹H-NMR）进行表征。综合使用主成分分析、正交偏最小二乘判别分析和单变量统计分析方法探讨积滞化热证引起的机体代谢表型的变化，鉴定及比较相关的代谢标志物。结果：与正常组比较，模型组的肛温、饮水量、饮食量等有明显改变（P<0.05）。在血清和尿液中分别筛选得到6种和7种与积滞化热证相关的代谢标志物。模型组大鼠机体代谢表型发生了明显的改变，脂质代谢、糖吸收和代谢、肠道菌群都受到不同程度的影响。清伙灵组与模型组比较差异有统计学意义（P<0.05）。结论：清伙灵能有效改善积滞化热证候大鼠的脂质和糖代谢失衡，并可调节肠道菌群。

Abstract:

Objective: To investigate the metabolic profile of stagnant heat syndrome in rats and the intervention effects of Qinghuoling. Method: There were control, model and Qinghuoling groups.Model and Qinghuoling groups were treated with special feed and ip atropine (0.25 mg·kg^-1),twice a day, continues modeling for 7 days, from the 4 th day of modeling, ig Qinghuoling(2.64 g·kg^-1),once a day.Proton nuclear magnetic resonance(¹H-NMR) technique was used to analyze the rat serum and urine metabolic profiles. Multivariate data analysis methods such as orthogonal partial least squares discriminant analysis (OPLS-DA) and principal components analysis (PCA), and univariate statistical analysis methods were used to analyze the metabonome difference between the control and model group samples, to identify the corresponding metabolic biomarkers and to study the intervention effect of Qinghuoling. The mechanism of action of Qinghuoling was illustrated through the selected biomarkers. Result: Compared with the control group, rectal temperature, water intake, and food intake of model group has a significant change The metabolic phenotype of rats in model group greatly changed, Lipid metabolism, glucose uptake and metabolism, intestinal flora have been affected in different degrees and Qinghuoling had efficacious intervention. Six and sevenpotential biomarkers were selected in rat serum and urine, respectively. Conclusion: Qinghuoling can effectively improve the disturbance of lipids and glucose metabolismand modulate the gut microflora environment in rats.