扎里奴思方对脑缺血再灌注损伤后大鼠脑内NGF表达的影响

Effects of Zhali Nusi Fang on Nerve Growth Factor Expression in Rat Brain after Cerebral Ischemia Reperfusion

摘要:

目的：观察扎里奴思方对脑缺血再灌注损伤后大鼠脑内神经生长因子（NGF）表达的影响。方法：线栓法制备局灶性脑缺血再灌注大鼠模型。实验动物随机分为假手术组、模型组、扎里奴思方组、尼莫地平组。取材前进行神经功能评分。术后各组分别在1，3，7，14 d各个时间点取材，HE染色法观察大鼠脑组织病理学改变；免疫组织化学法测定大鼠脑内NGF 的表达。结果：模型组大鼠神经功能评分降低，神经元数量减少，大小不均，病理损伤显著；与模型组比较，尼莫地平和扎里奴思方组神经评分增高，神经元数量增多，脑组织病理损伤减轻明显，NGF表达增高（P<0.01，P<0.05）；与尼莫地平组比较，扎里奴思方14 d组神经功能评分和NGF表达明显升高（P<0.05）。结论：扎里奴思方可对局灶性脑缺血再灌注大鼠脑组织神经元起保护作用，扎里奴思方14天组的脑保护作用更强，其脑保护作用机制可能与调节大鼠脑组织 NGF 表达有关。

Abstract:

Objective: To observe the effects of Zhali Nusi fang(ZLNSF) on nerve growth factor(NGF) expression in the rat brain after cerebral ischemia reperfusion. Method: Rat focal cerebral ischemia reperfusion model was established by middle cerebral artery occlusion (MCAO) with nylon thread.The animal models were randomized into sham-operation,model, nimodipine(NMDP) and ZLNSF 4 groups to receive treatment with normal saline, NMDP and ZLNSF 30 min after the reperfusion. To do neurological score before take material.To take material at 1,3,7,14 days.Immunohistochemistry was employed to detect the expressions of nerve growth factor at rat brain cortex. Result: The neurological scores of model group rats are reduce, the number of nerve cells are cut down with unequal size and pathological damage significant. compared with model groups, the neurological scores of ZLNSF and NMDP groups are raise, the number of nerve cells are raise, pathological damage brain tissue are reduce significantly and NGF expression are enhance (P<0.05). Compared with NMDP groups, the level of NGF expression was significantly higher in ZLNSF 14 days group (P<0.05). Conclusion: ZLNSF show protective effects against global ischemia-reperfusion injury,ZLNSF 14 days group has the highist cerebral protective effects, and its cerebral protection mechanism may be related to regulate NGF expression in rat brain.