抗柯萨奇B病毒性心肌炎胶囊复方新制剂对病毒性心肌炎小鼠血清TNF-α,IL-6的影响

Effect of New Formulation of K-CoxB-JN Compound on TNF-α and IL-6 in Mice with Coxsackie B3 Viral Myocarditis

摘要:

目的：探讨抗柯萨奇B病毒性心肌炎胶囊复方新制剂（K-CoxB-JN）对病毒性心肌炎小鼠血清肿瘤坏死因子-α（TNF-α）、白介素-6（IL-6）的影响及其机制。方法：用CVB3感染Balb/c小鼠造成病毒性心肌炎模型，随机分为5组：K-CoxB-JN复方新制剂低、中、高剂量组（0.2，0.4，0.8 g·kg^-1），利巴韦林组（0.075 g·kg^-1），模型组，并设正常组，鼠腹腔接种病毒2 h后开始灌胃，连续灌胃14 d。观察小鼠一般状态、记录死亡数，计算小鼠生存率；取血测定血清TNF-α，IL-6水平；取心脏，计算心脏指数，HE染色，计算病理积分。结果：与正常组比较，模型组小鼠血清TNF-α，IL-6水平、心脏指数、心脏病理积分均升高（P<0.05），生存率降低（P<0.05）；与模型组比较，K-CoxB-JN各组小鼠心脏指数，TNF-α，IL-6水平、心脏病理积分均降低（P<0.05），生存率升高（P<0.05）。结论：调节TNF-α，IL-6水平可能是K-CoxB-JN治疗病毒性心肌炎作用机制之一。

Abstract:

Objective: To investigate the effect and its mechanisms of the new formulation of K-CoxB-JN compound on tumor necrosis factor-α(TNF-α) and interleukine(IL)-6 in mice with coxsackie B3 viral myocarditis. Method: The mice were injected with CVB3 to establish the experimental model of viral myocarditis,then randomly divided into 5 groups:The new formulation of K-CoxB-JN compound low,middle,high dose group (0.2,0.4,0.8 g·kg^-1),ribavirin group(0.075 g·kg^-1), the model group.and set up the normal group,two houres later mice were gavaged for 14 days. The general state of the mice were observed, deaths were recorded and calculated the survival rate of mice;the content of TNF-α, IL-6 were tested. Took heart and calculated the cardiac index, HE staining,calculated the pathological integral. Result: Compared with the normal group,the model group mice cardiac index,TNF-α and IL-6 level, the pathological integral were increased(P<0.05), the survival rate were decreased(P<0.05),Compared with the model group, the new formulation of K-CoxB-JN compound group mice cardiac index,TNF-α and IL-6 level, the pathological integral were decreased(P<0.05), the survival rate were increased(P<0.05). Conclusion: Regulation of TNF-α, IL-6 level may be one of the mechanisms of the new formulation of K-CoxB-JN compound in the treatment of viral myocarditis.