两色金鸡菊醇提物对自发性高血压大鼠血压和血浆ET,AngⅡ,CGRP的影响

Effect of Alcohol Extract from Coreopsis tinctoria on Blood Pressure and Contents of ET, AngⅡ, CGRP in Spontaneous Hypertension Rats

摘要:

目的：观察新疆两色金鸡菊醇提物对自发性高血压大鼠（SHR）血压、心肌重构和血浆内皮素（ET）、血管紧张素（Ang）Ⅱ和降钙素基因相关肽（CGRP）含量的影响。方法：将SHR分为模型组、两色金鸡菊醇提物高、中、低剂量组（3.2，1.6，0.8 g·kg^-1）、卡托普利组（4 mg·kg^-1）及杜仲平压片组（187.5 mg·kg^-1），连续ig给药4周。采用无创血压计观察其对血压的影响；计算各组大鼠左心室质量指数（LVWI）和心脏指数；采用放射免疫法测量血浆中ET，AngⅡ，CGRP含量的变化。结果：干预4周后，各给药组大鼠血压均明显下降（P<0.01）；与模型组相比各组大鼠LVWI均下降，其中两色金鸡菊醇提物高剂量组和卡托普利组的下降存在显著差异（P<0.01），且高剂量组和卡托普利组心脏指数降低 （P<0.05）；与模型组相比，两色金鸡菊醇提物高、中剂量和卡托普利可明显减少血浆中ET和AngⅡ的含量（P<0.01），同时血浆中CGRP的含量有增加的趋势。结论：两色金鸡菊醇提物具有一定的降压作用，可以改善SHR心室肥厚情况，其作用机制与抑制体内肾素-血管紧张素系统和改善内皮功能有关。

Abstract:

Objective: To observe the effects of the alcohol extract from Coreopsis tinctoria on blood pressure, the content of plasma endothelin (ET), angiotension Ⅱ (AngⅡ) and calcitonin gene-related peptide(CGRP) in spontaneous hypertension rats (SHR). Method: The spontaneous hypertension rats (SHR) were randomly divided into six groups, untreated control,high, middle, low dosage group of the alcohol extract from C. tinctoria(3.2,1.6,0.8 g·kg^-1), captopril group(4 mg·kg^-1) and du zhong tablet group(187.5 mg·kg^-1). Tail blood pressure were measured every week.After 4 weeks, the contents of plasma ET, AngⅡ and CGRP were detected by radioimmunity method respectively. The left ventricular weight index (LVWI) and cardiac index were monitored too. Result: Compared with model group,the blood pressure of all groups significantly lowered after 4 weeks (P<0.01).The LVWI of all groups was decreased, while the high dosage group and captopril group showed significant difference (P<0.01).The contents of ET and AngⅡ were decreased greatly (P<0.01) in the high,middle dosage group and captopril group, while the contents of plasma CGRP were increased in all group,but showed no significant difference. Conclusion: The alcohol extract from C. tinctoria could significantly reduce blood pressure and improve myocardial hypertrophy in SHR,which might be related to inhibiting RAS and improving the endothelial function.