糖痹康对糖尿病大鼠坐骨神经NGF蛋白及NGF mRNA表达的影响

Effect of Chinese Herbal Compound Tangbikang on Expressions of NGF Protein and NGF mRNA of Diabetic Rats’ Sciatic Nerve

摘要:

目的：观察中药复方糖痹康对糖尿病大鼠坐骨神经神经生长因子（nerve growth factor ，NGF）蛋白及NGF mRNA表达的影响。方法：采用高脂饲料和小剂量链脲佐菌素（streptozotocin，STZ）诱发2型糖尿病大鼠动物模型，造模成功后随机分为模型组、甲钴胺组（1.97 mg·kg^-1）、糖痹康高、中、低剂量组（分别为生药4.175，8.35，16.7 g·kg^-1），另设10只雄性SD大鼠为正常组，模型组和正常组给予蒸馏水，用不同剂量的糖痹康灌胃，并与甲钴胺对照，16周后取大鼠一侧坐骨神经，用免疫组化法检测坐骨神经中NGF蛋白的表达及采用RT-PCR方法检测坐骨神经组织中NGF mRNA的表达。结果：与正常组比较，模型组NGF蛋白及NGF mRNA表达显著降低（P<0.01）；与模型组比较，糖痹康中、高剂量组能升高 NGF蛋白及NGF mRNA表达（P<0.01），糖痹康低剂量组能升高NGF mRNA表达（P<0.05）。结论：中药复方糖痹康能够促进糖尿病周围神经病变大鼠坐骨神经NGF mRNA的转录和蛋白的表达。

Abstract:

Objective: To explore the effect of Chinese herbal compound Tangbikang(TBK) on the expressions of nerve growth factor (NGF) protein and NGF mRNA of diabetic rats' sciatic nerve. Method: Streptozotocin(STZ) and feeding high fat forage induced diabetic rats were randomly divided into diabetic model group, Mecobalamin group, TBK high dose group, TBK middle dose group, TBK low dose group and SD normal male rats (n=10). Normal control group and model group were daily gavaged with distilled water, TBK groups were given TBK with different dose(4.175,8.35,16.70 g·kg^-1). Mecobalamin (1.97 mg·kg^-1) was used as the control medicine. Rats' unilateral sciatic nerve were taken out at the 16^th week, immunohistochemical method was used to detect the expressions of NGF protein of sciatic nerve, and the expressions of NGF mRNA were detected by semi-quantitative RT-PCR. Result: Compared with normal group, the expressions of NGF protein and NGF mRNA of model group were significantly decreased (P<0.01), Compared with the model group, high and middle dose TBK group significantly increased the expressions of NGF protein and NGF mRNA (P<0.01) and low dose TBK group significantly increased the expressions of NGF mRNA (P<0.05). Conclusion: Chinese herbal compound TBK could promote the expressions of NGF and NGF mRNA of diabetic peripheral neuropathy rats' sciatic nerve.