复方玉郎伞多糖对鸭乙型肝炎病毒诱导的肝损伤的影响

Effect of Compound Yulangsan Polysaccharide against Duck Liver Injury Induced by Duck Hepatitis B Virus

摘要:

目的：研究复方玉郎伞多糖（CYLSP）在鸭乙型肝炎病毒（DHBV）持续性感染模型中对DHBV所致的肝损伤的保护作用。方法：采用1 d龄雏鸭接种广西麻鸭乙型肝炎病毒（DHBV）强阳性血清，接种1周后用聚合酶链式反应（PCR）法筛选出DHBV强阳性鸭50只，随机分为5组，每组10只，CYLSP高、中、低剂量组（10，5，2.5 g·kg^-1）、拉米夫定（0.05 g·kg^-1）组和模型组，每日上午ig给药1次，连续14 d。于用药前（T₀）、用药7 d（T₇）和14 d（T₁₄）及停药后3 d（P₃）分别采血，同时检测血清中丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）的活性；在停药3 d后，取肝脏匀浆并除蛋白，检测肝匀浆液中总超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性以及谷胱甘肽（GSH）、丙二醛（MDA）的含量；HE染色处理肝脏组织切片，光镜观察病理学改变。结果：与同组T₀及模型组比较，各给药组鸭血清高、中剂量治疗组给药7 d （T₇）和14 d （T₁₄）即能明显降低血清ALT，AST的活性，停药3（P₃）天后，CYLSP高剂量组仍能显示出持续有效，没有出现反跳现象（P<0.05或P<0.01）；检测肝匀浆液中SOD，GSH-Px活性以及GSH，MDA的含量，CYLSP高、中剂量组和拉米夫定组仍能显示出持续有效，没有出现反跳现象（P<0.05或P<0.01）；HE染色显示CYLSP可显著减轻鸭肝组织病理损伤程度。结论：CYLSP对DHBV所致的肝损伤具有保护作用。

Abstract:

Objective: To study the inhibitory effect of compound Yulangsan polysaccharide (CYLSP) on duck hepatitis B virus (DHBV) persistent infection. Method: One-day old Guangxi brown spotted ducks infected with hepatitis B virus (DHBV) were used as the DHBV infected animal model. Fifty positive ducks were detected by PCR at one week after the infection of DHBV, then randomly divided into 5 groups, each group of 10, only CYLSP high, medium and low dose group (10,5,2.5 g·kg^-1) and lamivudine (0.05 g·kg^-1) group and model group, the daily morning lavage l time for 14 d. Alanine aminotransferase(ALT), aspartate aminotransferase(AST) in serum were detected before treatment (T₀),7 days (T₇),14 days (T₁₄) after treatment, and 3 days after stopping treatment. At 3 days after stopping treatment, the livers were collected,homogenate was prepared to detect superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) activity and reduced glutathion(GSH),malondialdehyde(MDA) levels, HE staining was used to observe the pathological change. Result: Compared with T₀ and model group, the serum levels of ALT and AST were decreased significantly and 3 days after stopping treatment,CYLSP high dose group and lamivudine group still showed a protection effect (P<0.05 or P<0.05) SOD,GSH-Px activity and the content of GSH,MDA.CYLSP in high, middle dose group and lamivudine group showed a protection action. In CYLSP low dose group, no change of SOD,GSH-Px activity and GSH,MDA content was found,HE staining showed CYLSP significantly reduced the duck liver histopathologic damage degree. Conclusion: The compound Yulangsan polysaccharide has a protection action on duck liver injury induced by duck hepatitis B virus