参芪花粉片对大鼠运动性疲劳模型的影响

Study of Shenqi Huafen Pian on Exercise-induced Fatigue in Rats

摘要:

目的：探讨参芪花粉片抗疲劳的作用特点。方法：将70 只雄性SD大鼠随机分为空白组、模型组、参芪花粉片高、中、低剂量组（2.5，1.25，0.625 g·kg^-1）、硝苯地平片组（0.01 g·kg^-1）、刺五加片组（0.25 g·kg^-1）。连续 ig 给药 9 d。采用 负重游泳实验造大鼠力竭模型。测定体重、肝脏中三磷酸腺苷酶（ATP）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、肾脏中丙二醛（MDA）、超氧化物歧化酶（SOD）、血乳酸（LD）等指标。结果：与空白组比较，模型组ATP，CAT，GSH-Px，MDA，SOD，血LD等指标显著降低（P<0.01）。高、中剂量参芪花粉片组体重均大于模型组（P<0.01）。与模型组相比，高剂量参芪花粉片组可明显改善肝脏Na⁺-K⁺-ATP酶、Mg²⁺-ATP酶和Ca²⁺-ATP酶活性（P<0.05），显著提高肝脏GSH-Px活性（928.4±150.1）U·mL^-1（P<0.01）和肾脏SOD活性（289.0±4.2）UN·mL^-1（P<0.01）；高、中剂量参芪花粉片组均可显著提高肝匀浆CAT活性（399.2±78.1），（372.0±139.9）U·g^-1（P<0.01），显著降低肾脏MDA水平（1.93±0.33），（2.18±0.47）nmol·mg^-1（P<0.01），明显降低血LD水平（7.84±1.18），（9.69±1.36）mmol·L^-1（P<0.05）。结论：参芪花粉片可显著增强大鼠的抗疲劳能力，其作用可能与抗氧化和加速乳酸代谢有关。

Abstract:

Objective: To study of Shenqi Huafen Pian on exercise-induced fatigue in rats. Method: Seventy male SD rats were randomly divided into normal control group,model control group,Shenqi Huafen Pian high,medium,low dose groups(2.5,1.25,0.625 g·kg^-1), Nifedipine tablets groups(0.01 g·kg^-1),Ciwujia tablets groups (0.25 g·kg^-1).Drugs were administered to mice for 9 days.Except control group,the other 6 groups were conducted exhaustive swimming experiment. The weight,MDA(malonaldehyde) and lactic acid(LD) content, adenosine triphosphatase(ATPase),catalase(CAT),Glutathione peroxidase(GSH-Px) and superoxidedismutase(SOD) activity were measured. Result: The weight in Shenqi Huafen Pian high,medium dose groups was increased(P<0.01).In high dose group of Shenqi Huafen Pian, Na⁺-K⁺-ATPase activity,Mg²⁺-ATPase and Ca²⁺-ATPase activity of liver cells were obviously increased compared with model(P<0.05),GSH-Px activity in liver was significantly higher than that in model group(P<0.01), activities of SOD in kidney were significantly higher than that(P<0.01).In high and middle dose group of Shenqi Huafen Pian, CAT activities in liver were significantly higher than that in model group(P<0.01), MDA content in kidney was lower than that(P<0.01), blood LD contents were obviously lower than that in model group(P<0.05). Conclusion: Shenqi Huafen Pian showed anti-fatigue effects.The effects could be mediated by increasing the ability of antioxidation and improving lactate metabolism.