鸭脚木皮中总三萜的提取工艺优化

Optimization of Extraction Technology for Total Triterpenoids from Bark of Schefflera octophylla

摘要:

目的：建立鸭脚木皮中总三萜的含量测定方法并优选其提取工艺。方法：采用香草醛-高氯酸-浓硫酸法测定总三萜含量，检测波长540 nm。以总三萜提取率为指标，通过正交试验考察料液比、提取时间和提取次数对鸭脚木皮中总三萜提取工艺的影响。结果：以齐墩果酸为对照品，线性范围0.083 2~0.280 02 mg，加样回收率99.36%。最佳提取工艺为加10倍量水煎煮提取3次，每次1 h；总三萜平均提取率2.94%，RSD 0.5%。结论：建立的总三萜含量测定方法稳定可靠，优选的提取工艺合理可行，为鸭脚木皮有效部位的开发提供参考。

Abstract:

Objective: To establish a determination method of total triterpenoids in bark of Schefflera octophylla and optimize its extraction technology. Method: Vanillin-perchloric acid-concentrated sulfuric acid method was adopted to determine the content of total triterpenoids with detection wavelength of 540 nm.Based on single-factor tests, L₉(3⁴) orthogonal test was designed to investigate effects of solid-liquid ratio, extracting time and times on extraction technology by taking yield of total triterpenoids as index. Result: With oleanolic acid as the reference substance, there was a good linear relationship in 0.083 2-0.280 02 mg, the average recovery was 99.36%.Optimal extraction process was as follows:extracted thrice with 10 times the amount of water, 1 h for each time;Under these conditions, average extraction ratio of total triterpenoids was 2.94% with RSD of 0.5%. Conclusion: This established method for determination of total triterpenoids was stable and reliable, optimized extraction technology was reasonable and feasible, which could provide theoretical basis for development of effective parts from bark of S. octophylla.