Box-Behnken响应面法优化芦笋总皂苷超声提取工艺

Optimization of Ultrasound Extraction Technology for Total Saponins from Asparagus officinalis by Box- Behnken Response Surface Methodology

摘要:

目的：优选芦笋总皂苷的超声提取工艺。方法：以总皂苷含量为指标，在单因素试验基础上，通过Box-Behnken响应面法考察乙醇体积分数、料液比、超声时间和温度对提取工艺的影响。结果：最佳超声提取工艺为加15倍量74%乙醇于50 ℃超声提取54 min；总皂苷平均质量分数13.059%（RSD 1.63%），与预测值（13.185%）的偏差较小。结论：优选的提取工艺稳定可行，为芦笋总皂苷的开发应用提供实验依据。

Abstract:

Objective: To optimize ultrasound extraction technology of total saponins from Asparagus officinalis. Method: With the content of total saponins as index, effects of ethanol concentration, solid-liquid ratio, ultrasonic time and temperature on extraction technology were investigated by single factor tests and Box-Behnken response surface methodology. Result: Optimum ultrasound extraction technology was as following:ultrasonic extracted 54 min with 15 times the amount of 74% ethanol at 50 ℃;Under these conditions, the average mass fraction of total saponins was 13.059%, which was wery close to the predicted value of 13.185%. Conclusion: This optimized technology was stable and feasible to provide a basis for development and application of total saponins in A. officinalis.