复方天麻钩藤口腔崩解片的半仿生提取工艺优选

Optimization of Semi-bionic Extraction Technology for Compound Tianma Gouteng Orally Disintegrating Tablets

摘要:

目的：优选复方天麻钩藤口腔崩解片的半仿生提取工艺。方法：以天麻素、钩藤总碱、芍药苷和阿魏酸提取量的综合评分为指标，采用正交试验考察加水量、提取时间、提取温度对提取工艺的影响。采用HPLC测定天麻素、芍药苷和阿魏酸含量，流动相分别为乙腈-0.05%磷酸溶液（3：97），乙腈-0.1%磷酸溶液（14：86），乙腈-0.1%磷酸溶液（17：83），检测波长分别为220，230，316 nm，运用UV测定钩藤总碱含量。结果：最佳提取工艺为加12倍量水于60 ℃提取2 h；5倍处方量药材中天麻素、钩藤总碱、芍药苷、阿魏酸提出总量分别为0.479，1.276，4.802，0.061 g。结论：优选的工艺条件稳定可行，适合复方天麻钩藤口腔崩解片的工业化生产。

Abstract:

Objective: To optimize semi-bionic extraction technology of compound Tianma Gouteng orally disintegrating tablets. Method: With composite score of gastrodin, rhomotoxine, paeoniflorin and ferulic acid extraction amounts as index, L₉(3⁴) orthogonal test was adopted to investigate effects of the amount of water, extracting time and temperature on semi-bionic extraction technology.HPLC was used to determine contents of gastrodin, paeoniflorin and ferulic acid, mobile phase were acetonitrile-0.05% phosphoric acid(3:97), acetonitrile-0.1% phosphoric acid(14:86) and acetonitrile-0.1% phosphoric acid(17:83), detection wavelength were 220, 230 and 316 nm, respectively;The content of rhomotoxine was determined by UV. Result: Optimum extraction technology was as follows:extracted 2 h with 12 times the amount of water at 60 ℃;Total extraction amounts of gastrodin, rhomotoxine, paeoniflorin and ferulic acid in five times the amount of prescriptions medicines were 0.479, 1.276, 4.802 and 0.061 g, respectively. Conclusion: This optimized extraction process was stable and feasible, which was suitable for industrial production of compound Tianma Gouteng orally disintegrating tablets.