棉酚的大孔吸附树脂纯化工艺优选

Optimization of Purification Process for Gossypol from Gossypium herbaceum by Macroporous Adsorption Resin

摘要:

目的：优选从棉子中分离棉酚的大孔吸附树脂纯化工艺。方法：采用静态吸附-洗脱与动态吸附-洗脱试验筛选 大孔树脂型号，通过单因素试验优选洗脱流速，利用正交试验考察上样液质量浓度、洗脱剂乙醇体积分数、上样液pH及洗脱液中乙醇体积分数对棉酚纯化工艺的影响。结果：选用H103型大孔吸附树脂，最佳纯化工艺为上样液中棉酚质量浓度90 mg·L^-1，上样液pH 7，上样液中乙醇体积分数40%，洗脱剂为80%乙醇；棉酚纯度由提取液的6.2%提高至洗脱液中83.6%，转移率76.7%。结论：优选的工艺稳定可行，H103型大孔树脂适用于棉酚的纯化。

Abstract:

Objective: To optimize purification process of gossypol from Gossypium herbaceum by macroporous adsorption resin. Method: Static and dynamic adsorption/desorption tests for gossypol were carried out to select proper macroporous resin, single factor test was adopted to select elution rate, other purification parameters were optimized by orthogonal test, such as pH of sample solution, ethanol concentration and so on. Result: H-103 resin was selected as the best one among 13 different resins, optimal process was determinded as follows:the concentration of gossypol in loading solution 90 mg·L^-1 with pH of 7, the concentration of ethnol in loading solution 40% and in eluting solution 80%;Purity of gossypol was enhanced from 6.2% in extract to 83.6% in eluant with transfer rate of 76.7%.After evaporation and recycle of solvent, then gossypol was obtained by recrystallization in a mixture solvent of acetone-acitic acid. Conclusion: H-103 resin was suitable for purification of gossypol from G. herbaceum.