膜分离法和醇沉法对清消口服液的纯化效果比较

Comparison of Purification Effect of Qingxiao Oral Liquid by Membrane Separation Technology and Alcohol Precipitation Method

摘要:

目的：比较膜分离法和醇沉法对清消口服液的纯化效果。方法：采用HPLC测定黄芩苷含量，流动相甲醇（A）-0.05%磷酸水（B）梯度洗脱（0~10 min，30%A；10~30 min，30%~50%A；30~45 min，50%~80%A），检测波长280 nm，考察膜分离样品及醇沉样品中出膏量、黄芩苷含量及保留量的差异。结果：膜分离样品和醇沉样品中黄芩苷保留率分别为92.01%，72.79%，出膏量分别为4.14，2.48 g。结论：膜分离法对有效组分黄芩苷的保留率较醇沉法高，但后者在除杂方面较前者好，样品外观在观察期内无明显差异。

Abstract:

Objective: To compare purification effect of Qingxiao oral liquid with membrane separation technology and alcohol precipitation method. Method: HPLC was adopted to determine the content of baicalin in Qingxiao oral liquid with mobile phase of methanol(A)-0.05% phosphoric acid solution(B) gradient elution(0-10 min, 30%A;10-30 min, 30%-50%A;30-45 min, 50%-80%A) and detection wavelength at 280 nm, differences of dry extract yield, content and retention rate of baicalin between membrane separation samples and alcohol precipitation samples were investigated. Result: Retention rates of baicalin in membrane separation and alcohol precipitation samples were 92.01% and 72.79%, the amount of dry extract were 4.14 and 2.48 g, respectively. Conclusion: Retention rate of effective ingredient(baicalin) with membrane separation method was higher than alcohol precipitation method, but the latter had a better capacity by comparing with the former, appearance of samples had no significant difference in the observation period.