UPLC测定参麦注射液中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的含量

Content Determination of Ginsenoside Rg1, Ginsenoside Re and Ginsenoside Rb1 in Shenmai Injection by UPLC

摘要:

目的：采用超高效液相色谱法分离测定参麦注射液中人参皂苷Rg₁、人参皂苷Re、人参皂苷Rb₁的含量。方法：采用ACQUITY UPLC HSS T3 （2.1 mm×50 mm，1.8 μm）色谱柱，流动相乙腈（A）-0.05%磷酸（B），梯度洗脱（0~5 min，19%A；5~12 min，19%~28%A；12~18 min，28%~40%A），流速0.3 mL·min^-1，检测波长203 nm，柱温25 ℃。结果：人参皂苷Rg₁、人参皂苷Re和人参皂苷Rb₁分别在0.020 96~0.209 6，0.017 64~0.176 4，0.023 88~0.238 8 g·L^-1与相应峰面积呈良好线性关系，平均加样回收率（n=6）分别为100.12%，100.08%，99.51%，RSD分别为1.60%，2.03%，1.15%。结论：与HPLC相比，UPLC在不影响分离效果的情况下可显著提高参麦注射液中人参皂苷Rg₁、人参皂苷Re、人参皂苷Rb₁的分析速度，改善分析效果，同时可减少溶剂的消耗。该方法简便、快捷、准确、重复性好，可代替HPLC法用于参麦注射液的多指标质量控制。

Abstract:

Objective: To establish a method to determine the content of ginsenoside Rg₁, ginsenoside Re and ginsenoside Rb₁ in Shenmai injection by ultra performance liquid chromatography(UPLC). Method: ACQUITY^® UPLC HSS T3 column (2.1 mm×50 mm, 1.8 μm)was used with mobile phase consisted of acetonitrile (A)-0.05% phosphoric acid solution (B) by gradient elution (0-5 min, 19%A;5-12 min, 19%-28%A;12-18 min, 28%-40%A) at a flow rate of 0.3 mL·min^-1;the wavelength of detector was 203 nm. Result: Ginsenoside Rg₁, ginsenoside Re and ginsenoside Rb₁had good linearity within the range of 0.020 96-0.209 6, 0.017 64-0.176 4, 0.023 88-0.238 8 g·L^-1 respectively. The average recoveries(n=6)were 100.12%, 100.08%, 99.51% and RSDs were 1.60%, 2.03%, 1.15% respectively. Conclusion: UPLC method may greatly improve the separation efficiency and analysis speed in the case of ginsenoside Rg₁, ginsenoside Re and ginsenoside Rb₁ in Shenmai injection while reducing the solvent consumption. As an alternative of conventional HPLC, UPLC is more convenient, rapid, accurate and excellent repeatability.