鸡矢藤多糖的分离纯化及体内抗菌活性

Separation and Purification of Antibacterial Polysaccharides from Paederia scandens in vivo

摘要:

目的：对鸡矢藤多糖进行分离纯化，寻找具有体内抗菌活性的多糖组分。方法：采用柱色谱技术对鸡矢藤粗多糖（PSP）进行分离纯化，对纯化多糖组分采用凝胶柱和聚丙烯酰胺凝胶电泳进行纯度鉴定，借助气相色谱法和红外光谱判断其部分结构信息，采用体内抗菌模型对所得多糖的活性进行研究。结果：从鸡矢藤中分离2个单一多糖组分PSP2a和PSP2b。PSP2a由葡萄糖、半乳糖、甘露糖、木糖和阿拉伯糖组成，其摩尔比为4.92：3.23：1.00：1.73：3.50，PSP2b由阿拉伯糖、半乳糖和葡萄糖组成，其摩尔比为：1.00：0.73：1.80。PSP2a和PSP2b中单糖主要以呋喃糖形式存在。体内抗菌模型表明，PSP，PSP2和PSP2a对感染小鼠均有不同程度保护作用，其中以PSP2a的效果最好。结论：鸡矢藤多糖中具有体内抗菌活性的成分主要为PSP2a。

Abstract:

Objective: The polysaccharide was separated and purified from Paederia scandens for antibacterial polysaccharide in vivo. Method: The crude polysaccharides were separated and purified by column chromatography, and then the homogeneous polysaccharides were identified by gel column chromatography and polyacrylamide disc gel electrophoresis. Their structure was analyzed by GC and IR. The activities of polysaccharides were studied by antibacterial model in vivo. Result: Two kinds of homogeneous polysaccharides, PSP2a and PSP2b, were obtained from P. scandens. PSP2a were consisted of glucose, galactose, mannose, xylose and arabinose in the molar ratio of 4.92:3.23:1.00:1.73:3.50.PSP2b were consisted of arabinose, galactose and glucose in the molar ratio of 1.00:0.73:1.80.PSP2a and PSP2b mainly contained furanose. The antibacterial model in vivo indicated that PSP, PSP2 and PSP2a could protect mice infected with the resistant strain of P. scandens, and protection of PSP2a was best. Conclusion: The antibacterial component among polysaccharides from P. scandens was mainly PSP2a.