通关藤化学成分HPLC指纹图谱研究

HPLC Fingerprint of Chemical Constituents of Marsdenia tenacissima

摘要:

目的：研究通关藤化学成分的指纹图谱。方法：采用HPLC以TC-C₁₈色谱柱（4.6 mm×150 mm，5 μm），乙腈-水为流动相进行梯度洗脱，检测波长210 nm，流速1.0 mL·min^-1，柱温25 ℃。结果：以ZP-7通关藤苷元B（[11α-O-(2-甲基丁酰基)-12β-O-乙酰基通关藤苷元B]）为参照物，建立了通关藤的指纹图谱，指认了17个共有峰，精密度和重复性中共有峰相对保留时间RSD<3%，每个产地色谱图中的共有峰的峰面积之和>90%，10批药材的相似度在0.75~0.98，存在一定差异。结论：该方法准确、重复性好，为通关藤质量评价和质量控制提供依据，也为通关藤进一步开发奠定基础。

Abstract:

Objective: Fingerprint analysis of chemical constituents of Marsdenia Tenacissima. Method: With the use of HPLC, TC-C₁₈ column(4.6 mm×150 mm, 5 μm), acetonitrile-water was used as the mobile phase with gradient elution, detection wavelength at 210 nm, flow rate of 1.0 mL·min^-1, temperature of column at 25 ℃. Result: The fingerprint of M. tenacissima was established using ZP-7tenacigenin B [11α-O-(2-methylbutyryl)-12β-O-acetyltenacigenin B] as reference substance. 17 common peaks were identified. The precision and repeatability of the relative retention time RSD were less than 3%, each producing area in the chromatogram of common peak area was more than 90%. 10 batches of similarity were between 0.75-0.98, there were some differences. Conclusion: The method is accurate and reproducible, which can provide the basis for quality evaluation and quality control of M. tenacissima, also lay the foundation for further development of M. tenacissima.