人工种植红景天不同药用部位中红景天苷的含量测定

Study on Qualtaive Method of Salidroside of Artifical Cultivation Ultivation Rhodiola Rosea by HPLC-MS

摘要:

目的：测定人工种植红景天不同药用部位花瓣、茎、根的含量。方法：取各1 g样品经过60%的乙醇溶解，超声波清洗器清洗50 min，过滤，旋蒸，加2 mL的甲醇，用微孔滤膜（0.45 μm）过滤，采用CAPCELL PAK C₁₈柱（2.0 mm× 150 mm，i.d.，3 μm），柱温 30 ℃，波长278 nm；样品温度10 ℃，进样体积10 μL。流动相为甲醇-1%乙酸溶液 45：55，流速为1 mL·min^-1进行定量分析。结果：有效部位中的红景天苷含量从高到低依次为花瓣（35.00mg）、根（18.89mg）、茎（1.59 mg）。结论：建立了红景天中红景天苷含量的高效液相色谱方法，可适用于不同药材中红景天苷含量的测定。

Abstract:

Objective: To determine the petals, stems, roots planted content Rhodiola different medicinal parts。Method: Each 1 g sample is dissolved after 60% ethanol, ultrasonic cleaner cleaning 50 min, filtered, rotary evaporated, add 2 mL of methanol, microporous filter membrane (0.45 μm) filter using CAPCELL PAK C₁₈ column ((2.0 mm× 150 mm, i.d., 3 μm ), column temperature 30 ℃, wavelength 278 nm, sample temperature 10 ℃, injection volume of 10 μL. The mobile phase was methanol-1% acetic acid solution 45:55, flow rate of 1 mL·min^-1 for quantitative analysis. Result: The effective parts of salidroside content in the order of the petals (35.00 mg), root (18.89 mg), stems (1.59 mg). Conclusion: A detection of salidroside content in Rhodiola rosea was established by high performance liquid chromatography. It can be applied to the analysis on the salidroside in the different medicinal materials.