一测多评法测定银杏叶提取物中7种黄酮类成分含量

A Quantitative Method for Simultaneous Assay of Seven Flavonoids Ingredients with One Marker in Ginkgo biloba Extract by QAMS

摘要:

目的：建立一测多评法测定银杏叶提取物中7种黄酮类成分的含量，验证方法在银杏叶提取物中应用的准确性和可行性。方法：以槲皮素为内参物，建立槲皮素与其他6种成分相对校正因子，计算其他6种成分的含量，实现一测多评。采用外标法测定银杏叶提取物中7个黄酮类成分的含量，Pearson相关系数法比较计算值和测定值的差异。结果：一测多评法的计算值和外标法测定值间无显著差异，实验所得的校正因子可信。结论：在对照品缺乏的情况下，以外标法测定槲皮素，利用相对校正因子实现对其他6种黄酮类成分含量测定可行。

Abstract:

Objective: To establish a quantitative method of multi-components by single marker(QAMS) for determining of seven flavonoids for the improvement of the quality control of Ginkgo biloba extract, as well as ensure the accuracy and feasibility of the method apply to G. biloba extract. Method: With the typical composition quercetin as the indexes, the relative correction factors(RCF) between the quercetin and the other six flavonoids were established to detect the quantitation of quercetin and calculate the quantitation of the other six constituents. The external standard method was used for quantitating the seven constituents, and contrasted calculated value with estimated value by Pearson correlation coefficient. Result: Calculated value and estimated value did not make significant difference on QAMS and external standard method. And it showed that the RCF was authentic. Conclusion: In the case of lacking reference substance, the quantitation of quercetin could be detected. It is feasible for taking advantage of the RCF to quantitate seven flavonoids.