白头翁皂苷D与不同血浆蛋白的结合率测定

Determination of Plasma Protein Binding Rate of Pulsatilla Saponin D in Several Species

摘要:

目的：研究白头翁皂苷D与不同血浆蛋白的结合率。方法：采用HPLC与平衡透析法相结合的方法测定白头翁皂苷D与牛血清、人血浆及大鼠血浆中蛋白的结合率，COSMOSIL 5C₁₈-MS-Ⅱ色谱柱（4.6 mm×250 mm，5 μm），流动相甲醇-水-甲酸（80：20：0.02），检测波长203 nm。结果：白头翁皂苷D质量浓度为0.06，0.11，0.25 g·L^-1时与牛血清白蛋白的结合率分别为（78.45±0.89）%，（77.61±1.14）%，（77.16±0.29）%，与人血浆蛋白的结合率分别为（68.91±0.54）%，（67.68±0.87）%，（67.88±0.71）%，与大鼠血浆蛋白的结合率分别为（78.15±0.76）%，（78.61±0.97）%，（78.24±0.93）%。结论：建立的白头翁皂苷D蛋白结合率测定方法简便、稳定、可靠。白头翁皂苷D是一种中等程度的蛋白结合药物，结合率不具有质量浓度依赖性。

Abstract:

Objective: To investigate different plasma protein binding rates of pulsatilla saponin D. Method: HPLC and equilibrium dialysis method were adopted to determine plasma protein binding rates of pulsatilla saponin D in bovine serum, human and rat plasma, COSMOSIL 5C₁₈-MS-Ⅱ column(4.6 mm×250 mm, 5 μm) was employed with mobile phase of methanol-water-formic acid(80:20:0.02) and detection wavelength at 203 nm. Result: Plasma protein binding rates of pulsatilla saponin D at three various concentrations (0.06, 0.11, 0.25 g·L^-1) were (78.45±0.89)%, (77.61±1.14)%, (77.16±0.29)% in bovine serum albumin, they were (68.91±0.54)%, (67.68±0.87)%, (67.88±0.71)% in human plasma and (78.15±0.76)%, (78.61±0.97)%, (78.24±0.93)% in rat plasma, respectively. Conclusion: This method for determining plasma protein binding rates of pulsatilla saponin D was simple, stable and reliable.Pulsatilla saponin D was a drug with moderate plasma protein binding rate, which did not have concentration-dependent manner.