帕珠丸对慢性乙醇性肝损伤大鼠PPAR-α1 与AMPK-α1的影响

Impact of Pazufloxacin Pill on PPAR-α1, AMPK-α1 in Rats with Chronic Alcoholic Liver Injury

摘要:

目的：观察帕珠丸对乙醇性肝损伤大鼠肝组织中过氧化物酶体增殖物激活受体α1 （PPAR-α1）及腺苷酸活化蛋白激酶α1（AMPK-α1）表达水平的影响及探讨其对肝脏的保护作用。方法：将78只纯系雄性SD大鼠随机分成正常对照组、模型对照组、联苯双酯组、帕珠丸低、中、高剂量组6组。模型组、联苯双酯组、帕珠丸低、中、高剂量组分别给予56%红星二锅头酒10 mL·kg^-1灌胃；正常组给予10 mL·kg^-1生理盐水灌胃，每天上午1次，连续灌胃12周，复制乙醇性肝损伤模型。造模成功后，帕珠丸低、中、高剂量组分别给予10 mL·kg^-1帕珠丸混悬液灌胃，剂量依次为0.05，0.10，0.20 g·kg^-1；联苯双酯组给予10 mL·kg^-1联苯双酯滴丸混悬液灌胃，剂量为0.003 g·kg^-1；正常对照组、模型对照组分别给予同体积生理盐水灌胃，各组每天上午灌胃1次，连续灌胃3周。检测血清丙氨酸转氨酶（ALT）、天门冬氨酸转氨酶（AST）的活性及甘油三酯（TG）的水平，并检测肝组织中PPAR-α1 mRNA、AMPK-α1 mRNA的表达水平。结果：与正常组比较，模型组大鼠血清肝脏湿重和肝指数以及ALT，AST，TG，均显著升高（P<0.05），模型组大鼠肝组织中PPAR-α1 mRNA，AMPK-α1 mRNA显著降低（P<0.01）。与模型组比较，帕珠丸各剂量组与联苯双酯组肝脏湿重和肝指数及ALT，AST，TG均显著降低（P<0.05）；与模型组比较，帕珠丸各剂量组与联苯双酯组PPAR-α1 mRNA，AMPK-α1 mRNA均显著升高（P<0.05）。结论：帕珠丸可上调肝组织内PPAR-α1 mRNA，AMPK-α1 mRNA的表达，提示该药对乙醇性肝病有一定的保护作用。

Abstract:

Objective: To observe the impact of Pazufloxacin pill on alcoholic liver injury in rats with peroxisome proliferators activator receptors alphα 1(PPAR-α1), AMP acitvated kinase-α1(AMPK-α1) and explore its protective effect on the liver. Method: The 78 inbred male SD rats were randomly divided into normal control group, model group, diphenyl dimethyl bicarboxy late(DDB) group, Pazufloxacin pill low, medium and high dose groups 6 groups. Model group, DDB group, Pazufloxacin pill low, medium and high dose groups were given 56% of the Red Star Erguotou wine 10 mL·kg^-1 gavage;given normal saline 10 mL·kg^-1, every morning a second consecutive gavage 12 weeks;to copy ethanol-induced liver injury model. After the modeling, Pazufloxacin pills low, medium and high dose groups were given 10 mL·kg^-1 Pazufloxacin pill suspension gavage, Doses were 0.05, 0.10, 0.20 g·kg^-1;DDB group was given 10 mL·kg^-1suspension drop pills orally, at a dose of 0.003 g·kg^-1;normal control group, model control group were given the same volume of saline, administered once a day in each group, continuous gavage three weeks. The serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity and triglyceride (TG) levels were measured, and liver tissue PPAR-α1 mRNA, AMPK-α1 mRNA expression levels were detected. Result: Compared with normal group, model group, serum liver wet weight and liver index and ALT, AST, TG, were significantly increased (P<0.05), model group rat liver tissue PPAR-α1 mRNA, AMPK-α1 mRNA was significantly lower (P<0.01). Compared with model group, in Pazufloxacin pill each dose group and the DDB group liver wet weight and liver index and ALT, AST, TG levels were significantly lower (P<0.05);Compared with model group, in Pa beads pill each dose group and DDB group PPAR-α1 mRNA, AMPK-α1 mRNA were significantly increased (P<0.05). Conclusion: Pazufloxacin pill can increase the liver tissue PPAR-α1 mRNA, AMPK-α1 mRNA expression.It is pointed that the drugs have a protective effect on treating alcoholic liver disease.