复方血栓通胶囊抗血栓作用的实验研究

Study on Antithrombotic Effect of Fufang Xueshuantong Capsule

摘要:

目的：研究复方血栓通胶囊的抗血栓作用。方法：将大鼠随机分为模型对照组、复方血栓通胶囊低、中、高剂量组（0.375，0.75，1.5 g·kg^-1·d^-1）、阿司匹林肠溶片组（50 mg·kg^-1·d^-1），灌胃给药，每天1次，连续7 d。末次给药1 h后，建立大鼠下腔静脉血栓模型和动-静脉旁路血栓模型，观察复方血栓通胶囊对血栓湿重、干重及血栓形成抑制率的影响；将大鼠按上述方法分组，建立颈总动脉血栓溶栓模型，从造模当天开始灌胃给药，每天1次，连续5 d，末次1 h后取大鼠血栓，观察复方血栓通胶囊对血栓湿重及溶栓率的影响；将大鼠随机分为正常对照组、模型对照组、复方血栓通胶囊低、中、高剂量组、阿司匹林肠溶片组，采用皮下注射盐酸肾上腺素联合冰浴、减少喂食的方法建立大鼠急性血瘀模型，造模期间同时灌胃给药，每天1次，连续7 d，第8 d造模成功，灌胃给药1 h后，观察复方血栓通胶囊对大鼠凝血时间（CT）、凝血酶时间（TT）及血小板聚集功能的影响。结果：与正常组比较，模型组CT，TT显著缩短（P<0.01），血小板聚集能力显著提高（P<0.01）；与模型组比较，复方血栓通胶囊组大鼠下腔静脉、动-静脉旁路血栓的湿重和干重减轻（P<0.05~P<0.01），颈总动脉血栓的湿重减轻（P<0.05），CT和TT延长 （P<0.05~P<0.01），血小板聚集能力降低（P<0.05）。结论：复方血栓通胶囊具有抗血栓作用，其机制可能与改善凝血功能、血小板聚集功能有关。

Abstract:

Objective: To study the antithrombotic effect of Fufang Xueshuantong capsule. Method: The rats were randomized into model group, low, moderate and high dosages(0.375, 0.75, 1.5 g·kg^-1·d^-1) groups of Fufang Xueshuantong capsule and aspirin enteric-coated tablets(50 mg·kg^-1·d^-1) group, each group was ig administrated once every day for 7 days. One hour after the last administration, the vena cava inferior thrombus model and carotid arterio-venous bypass thrombus model were built, the effect of Fufang Xueshuantong capsule on wet weight and dry weight of thrombus, and thrombogenesis-inhibition rate in rats were observed. The carotid artery thrombus model wasestablished, meanwhile intragastric administration lasted 5 days. After the last administration, the effect of Fufang Xueshuantong capsule on wet weight and thrombogenesis-inhibition rate in rats were observed. The rats were given subcutaneous injection of epinephrine, ice-bath and reducing feeding was used to establish acute blood stasis model.The effect of Fufang Xueshuantong capsule on clotting time(CT), thrombin time(TT), and platelet aggregation function of rats was observed. Result: Compared with normal group, CT and TT of model group were decreased (P<0.01), the function of platelet aggregation was increased (P<0.01).Compared with model group, Fufang Xueshuantong capsule could decrease wet weight and dry weight of vena cava inferior thrombus and carotid arterio-venous bypass thrombus, reduce wet weight of carotid artery thrombus (P<0.05, P<0.01), prolong CT and TT (P<0.05, P<0.01), and inhibit platelet aggregation of rats (P<0.05). Conclusion: Fufang Xueshuantong capsule exhibits an evident antithrombotic effect probably through conditioning the coagulation function and platelet aggregation function.