灵巴菌质油对A549细胞成瘤能力及伪足形成的影响

Effect of LBJZ Oils on Tumor Growth and Pseudopodium Formation of A549 Cells

摘要:

目的：探讨灵巴菌质油对人肺癌细胞A549成瘤能力及伪足形成的效应。方法：灵巴菌质油25，50，100 mg·L^-1分别作用A549细胞20 d和24 h，通过软琼脂克隆形成法和PI单染法观察灵巴菌质油对A549细胞的成瘤能力和生长周期的影响；灵巴菌质油100 mg·L^-1作用A549细胞24 h，通过免疫荧光染色法观察细胞骨架的影响。结果：灵巴菌质油能够抑制细胞克隆的形成，低、中、高剂量组作用A549细胞20 d后的平均细胞克隆形成数与空白对照组比较均有极显著差异（P<0.01），抑制率与药物浓度呈正相关，并将细胞的生长阻滞在G₂期，中、高剂量组G₂期细胞比例分别为21.92%和29.94%，与空白对照组12.44%相比均有显著性差异（P<0.05）；但灵巴菌质油能改变A549细胞中微丝结构的分布，使其产生伪足。结论：灵巴菌质油具有降低A549细胞克隆形成能力及损伤其细胞骨架的作用，但它亦可能导致瘤细胞的迁移。

Abstract:

Objective: To investigate the effect of LBJZ oils on tumor growth and pseudopodium formation of human lung cancer cell line A549 cells. Method: Cell tumorigenicity was assayed by soft agar colony formation after treating A549 cells with LBJZ oils of 25, 50, 100 mg·L^-1 for 20 d. The cell cycle was analyzed by flow cytometry after treating A549 cells with LBJZ oils of 25, 50, 100 mg·L^-1 for 24 h. The changing of cytoskeletal was detected by immunofluorescence dyeing after treating A549 cells with LBJZ oils of 100 mg·L^-1 for 24 h. Result: LBJZ oils inhibited proliferation of A549 cells in a dose-dependent manner. There were significant differences between results of colony formation in control group and in all three dosage groups (P<0.01). LBJZ oils blocked cell cycle progression at G₂ phase. Ratios of cells in G₂ phase of medium and high dosage groups were 21.92% and 29.94% respectively, which showed significant differences compared with that of control group (P<0.05). LBJZ oils caused damage to the cytoskeleton of A549 cells, and promoted generation of pseudopodia. Conclusion: It suggested that LBJZ oils significantly inhibited proliferation of A549 cells, which may related to arrest of cell cycle and changes of cytoskeleton on A549 cells. However, it can also lead to cell migration and cancer.