苍朴口服液的药效学实验研究

Experimental Study on Pharmacodynamics of Cangpo Oral Liquid

摘要:

目的：验证苍朴口服液的止泻、涩肠、抗炎、镇痛及免疫功效。方法：50只小鼠随机分为5组，苍朴口服液低、中、高剂量组分别按5，10，20 g·kg^-1灌胃给药，分别采用番泻叶致小鼠腹泻模型，二甲苯致小鼠耳肿胀模型、热板实验、巨噬细胞吞噬鸡红细胞实验观察苍朴口服液的药效作用。结果：与对照组比较，苍朴口服液能减少番泻叶致小鼠腹泻的湿粪数，给药后2 h内作用最明显（P<0.05，P<0.01）；能缩短碳末在肠道内的推进距离 （P<0.01）；能抑制耳廓肿胀程度（P<0.05，P<0.01），抑肿率略低于醋酸泼尼松；能不同程度的延长小鼠舔足反应时间（P<0.05，P<0.01），提高小鼠痛阈；能明显增强"脾虚"动物腹腔巨噬细胞的吞噬作用（P<0.01），呈量效关系。结论：苍朴口服液具有止泻、涩肠、抗炎、镇痛和增强免疫的作用，与临床功效相符。

Abstract:

Objective: To assess the anti-diarrhea, astringing intestine, analyesia anti-nociception and immunity pharmacodynamics of Cangpo oral liquid. Method: Fifty mice were randomly divided into five groups:control group(normal saline), positive control group and low, middle, high dosage groups of Cangpo oral liquid (5, 10, 20 g·kg^-1). The effect on the diarrhea induced by Senna leaf was evaluated by the amount of mouse wet dejecta. The effect on the propulsive motility of small intestine was evaluated by push distance of the charcoal powder in intestine. Xylene-induced mouse ear edema was conducted to assess the anti-inflammatory effect of the liquid. Hot plate test were conducted to assess the anti-nociception effect of the liquid. Immunity function was investigated by the phagocytes' phagocytosis of chicken RBC on animal model of spleen deficiency caused by Rhubarb. Result: Compared with the control group, Cangpo oral liquid reduced the amount of mouse wet dejecta (P<0.01, P<0.05), the most significant effect happened in 2 hours after administration. Cangpo oral liquid debased push distance of the charcoal powder in intestine, the high-dose group played the strongest role in three groups (P<0.01). Cangpo oral liquid could inhibit the mice auricle swelling by xylene (P<0.05, P<0.01), and acetate prednisone showed slightly stronger effect than the liquid. Cangpo oral liquid also delayed the latencies of licking paws and prolonged pain threshold in hot plate pain test in mice (P<0.05, P<0.01). In addition, the liquid improved the phagocytic index of peritoneal macrophage in animal pathological model of spleen deficiency caused by Rhubarb (P<0.01), and it showed a dosage-effect relationship. Conclusion: Cangpo oral liquid has the functions of anti-diarrhea, astringing intestine, anti-inflammatory, anti-nociception, and immunity effect, which provides the pharmacodynamic basis for treating calf spleen deficiency diarrhea in clinic.