基于文献研究的变应性鼻炎豚鼠模型造模评价方法的研讨

Allergic Rhinitis Model Evaluation Method Based on Literature Research

摘要:

目的：对以变应性鼻炎模型豚鼠为研究对象的文献进行分析，总结模型评价方法，寻找最佳的模型评价方法。方法：检索中国医院知识总库（CHKD），以“变应性鼻炎”和“豚鼠”或“过敏性鼻炎”和“豚鼠”为检索词，检索范围为1979~2013年，对文献中采用的模型评价方法进行分析。结果：检索共得到208篇文献，其中共计51篇文献在造模后描述了模型评价方法，即分别通过行为学积分、鼻黏膜分泌物涂片、被动皮肤过敏试验（PCA）、病理观察等方法评价模型。结论：各种评价方法中，行为学积分评价方法最常用，目前以赵秀杰提出的方法较为可靠，但操作细节无统一标准。在此基础上结合鼻黏膜分泌物涂片、PCA评价模型更肯定，但PCA和病理学观察需增加实验动物数量，不易操作。因此，目前的文献基础尚不能为最佳模型评价方法提供充分支持。

Abstract:

Objective: Analyzing the literature on guinea pig usage for allergic rhinitis research, in order to determine the best evaluation model. Method: China Hospital Knowledge Database (CHKD) was used to search the terms ‘allergic rhinitis’ and ‘guinea pig’ in the period between 1979 and 2013. Result: A total of 208 articles were found, of which 51 articles described the model evaluation methods, which included behavior score, nasal secretions smear, passive cutaneous anaphylaxis (PCA), and pathological observation. Conclusion: Behavioral score evaluation method is the most commonly used, currently the method proposed by Zhao is more fairly reliable, but there is not uniform standard on procedure. On this basis, combined with nasal secretions smear, and PCA evaluation model is more reliable, but for PCA and pathology observation there is the need to increase the number of animals, thus making it difficult to operate. Therefore, the current literature still does not provide adequate support for the best model evaluation method.