卡波姆对淫羊藿总黄酮酶解效果的影响

Effect of Carbomer on Enzymolysis of Total Flavonoids from Epimedii Folium

摘要:

目的：考察生物黏附剂卡波姆对淫羊藿总黄酮酶解效果的影响。方法：将淫羊藿总黄酮与卡波姆混合，加入蜗牛酶酶解，采用HPLC测定酶解液中朝藿定A、朝藿定B、朝藿定C和淫羊藿苷等主要黄酮成分及箭藿苷A、箭藿苷B、鼠李糖基淫羊藿次苷Ⅱ、宝藿苷Ⅰ和淫羊藿苷元等主要酶解产物的含量变化，并与未加入卡波姆时淫羊藿总黄酮的酶解过程比较。结果：在37 ℃，含1%卡波姆的缓冲溶液中，淫羊藿总黄酮中朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的酶解速率变慢，约需4 h才能完全酶解转化成次级苷，所需时间为无卡波姆时的2倍；酶解液中宝藿苷Ⅰ质量浓度始终高于未加卡波姆时的，最高量48.43 mg·L^-1，6 h时趋于稳定时12.99 mg·L^-1，最高量和稳定量分别为未加卡波姆酶解时的2.2，7.2倍；2~6 h内箭藿苷A和鼠李糖基淫羊藿次苷Ⅱ质量浓度都略高于未加卡波姆时的；之后次级苷被继续水解生成苷元，且苷元生成速率基本相同，约7.60 mg·L^-1·h^-1。结论：卡波姆会减缓淫羊藿总黄酮的酶解速率，促进次级苷的生成，对苷元的生成基本无影响。

Abstract:

Objective: To investigate effect of carbomer on enzymolysis of total flavonoids from Epimedii Folium. Method: Total flavonoids from Epimedii Folium and carbomer was mixed, then hydrolyzed by snailase, HPLC was adopted to observe content change of main flavonoids (including epimedin A, epimedin B, epimedin C, icariin and so on) and main hydrolysates (sagittatoside A, sagittatoside B, 2"-O-rhamnosylicariside Ⅱ, baohuoside Ⅰ and icaritin in enzymolysis liquid, and compared with enzymatic process (no carbomer) of total flavonoids from Epimedii Folium. Result: In a buffer solution (containing 1% carbomer, 37 ℃), hydrolysis rates of epimedin A, epimedin B, epimedin C and icariin were slower, it need about 4 h to completely transformed to secondary glycosides, which was twice in comparison with absense of carbomer;The content of baohuoside Ⅰ was always higher and contents of sagittatoside A and 2"-O-rhamnosylicariside Ⅱ was also higher during 2-6 h than absense of carbomer.The highest content of baohuoside Ⅰ was 48.43 mg·L^-1 and the final stable content was 12.99 mg·L^-1, which were 2.2 and 7.2 times as many as absense of carbomer, respectively.Afterwards, secondary glycosides could continue to be hydrolyzed to aglycone, their production rates were almost the same, which were about 7.60 mg·L^-1·h^-1. Conclusion: Carbomer could slow down enzymolysis rates of total flavonoids from Epimedii Folium, promote production of secondary glycosides, but had no effect on production of aglycone.