表面活性剂协同微波提取金荞麦总黄酮的工艺优选

Optimization of Surfactant Assisted Microwave Extraction Technology of Total Flavonoids from Fagopyri Dibotryis Rhizoma

摘要:

目的：优化金荞麦中总黄酮的提取工艺。方法：以微波功率、十二烷基硫酸钠质量分数、料液比和微波辐射时间为自变量，总黄酮提取量为因变量，通过对自变量各水平的多元线性回归，采用星点设计-响应面法优选提取工艺并进行预测分析。结果：最佳工艺条件为微波频率175 W，十二烷基硫酸钠质量分数1.28%，料液比1：21 g·mL^-1，微波辐射时间3.6 min；金荞麦中总黄酮提取量达1.06 mg·g^-1，与热回流法、微波法、超声法相比，总黄酮提取量分别提高了35%，14%，21%。结论：该工艺简便可行，具有省时节能、提取率高等优点，适用于金荞麦总黄酮的提取。

Abstract:

Objective: To optimize surfactant assisted microwave extraction process of total flavonoids from Fagopyri Dibotryis Rhizoma. Method: Taking microwave power, the concentration of sodium dodecyl sulfate (SDS), solid to liquid ratio and microwave extraction time as independent variables, extraction amount of total flavonoids as dependent variable, linear or nonlinear mathematic models were used to estimate relationship between independent variables and dependent variable, central composite design-response surface methodology were adopted to optimize extraction process, and prediction was carried out through comparing the observed and predicted values. Result: Optimum extraction conditions were as follows:microwave power 175 W, the concentration of SDS 1.28%, solid-liquid ratio of 1:21 g·mL^-1, microwave extraction time of 3.6 min;Under these conditions, yield of total flavonoids could reach 1.06 mg·g^-1.Compared with thermal reflux method, microwave method and ultrasonic method, extraction amount of total flavonoids with surfactant assisted microwave extraction process could increased 35%, 14% and 21%, respectively. Conclusion: Optimized process was simple and feasible, it had advantages of energy-saving, time-saving and high extracting rate, which could be used for extraction of total flavonoids from Fagopyri Dibotryis Rhizoma.