正交设计法优选祖师麻软化切制工艺

Optimization of Softening Cutting Process of Daphne giraldii by Orthogonal Design

摘要:

目的：优选祖师麻的软化切制工艺。方法：以祖师麻甲素、伞形花内酯、总香豆素、紫丁香苷提取量为指标，采用L₉（3⁴）正交试验考察加水量、润制时间和饮片切制厚度对软化切制工艺的影响。采用HPLC测定祖师麻甲素、伞形花内酯及紫丁香苷含量，利用UV测定总香豆素含量。结果：最佳软化工艺组合为加水0.8倍量润制0.5 h，切丝5 mm；祖师麻甲素、伞形花内酯、紫丁香苷、总香豆素提取量分别为1.058，0.669，5.793，3.640 mg·g^-1。结论：优选的工艺条件稳定可行，为祖师麻的工业化生产和质量控制提供工艺参数。

Abstract:

Objective: To optimize softening cutting process of Daphne giraldii. Method: With extraction amounts of daphnetin, umbelliferone, total coumarins and syringin as indexes, effects of adding water content, softening time and cutting thickness on softening cutting process were investigated by orthogonal design.Contents of daphnetin, umbelliferone and syringin were determined by HPLC, but the content of total coumarins was determined by UV. Result: The best softening cutting process was as follows:adding water amount 0.8 times, softening time 0.5 h and cutting thickness 5 mm;Extraction amounts of daphnetin, umbelliferone, syringin and total coumarins were 1.058, 0.669, 5.793, 3.640 mg·g^-1, respectively. Conclusion: This optimized process was stable and feasible, which could provide process parameters for industrial production and quality control of D. giraldii.