鹿角胶中氨基酸类成分的HPLC指纹图谱

HPLC Fingerprints of Amino Acids Constituents in Cervil Cornus Colla

摘要:

目的：建立鹿角胶氨基酸类成分的高效液相色谱指纹图谱。方法：采用异硫氰酸苯酯（PITC）柱前衍生法建立高效液相指纹图谱，梯度洗脱进行分离，选用Diamonsil C₁₈（2）（4.6 mm×250 mm，5 μm）色谱柱，流动相为乙腈-醋酸钠缓冲液（pH 6.5），检测波长254 nm，柱温43 ℃，流速1.0 mL·min^-1。结果：共指认13个共有峰，10批样品具有较高的相似度，其液相色谱指纹图谱可反映鹿角胶所含的18种氨基酸成分。结论：该方法稳定可靠，可为鹿角胶中氨基酸成分质量的全面控制提供依据。

Abstract:

Objective: To establish a method of fingerprint analysis of the amino acids on Cervil Cornus Colla by HPLC. Method: Cervil Cornus Colla was derivated by Phenyl isothiocyanate (PITC). The Chromatographic fingerprints were obtained by the Diamonsil C₁₈ (2) column (4.6 mm×250 mm, 5 μm) with the gradient elution solvent system composed of acetonitrie-natrium aceticum buffer solution (pH 6.5). The detective wavelength was set at 254 nm, the flow rate was 1 mL·min^-1 and the column temperature was maintained at 43 ℃. Result: The result showed that the amino acids constituents had a high similarity. 18 kinds of amino acids in Cervil Cornus Colla were detected, of which 13 peaks were identified in common peaks. Conclusion: The method has good reproducibility and stability, and could be used for the research and the quality control of Cervil Cornus Colla.