周氏克金岩方中6种黄酮指标成分的UV在线检测

UV on-line Detection of Six Flavonoids Index Ingredients in Zhou’s Prescription

摘要:

目的：建立周氏克金岩方中6种黄酮指标成分的快速UV在线检测方法。方法：用HPLC测定39批周氏方提取液中儿茶素、羟基红花黄色素A、金丝桃苷、异槲皮苷、槲皮苷、槲皮素6种黄酮指标成分含量；同时将这39批提取液在200~500 nm进行UV全波长扫描，取每隔1 nm的紫外吸光度。分别将HPLC测得各提取液6指标成分含量与其301个紫外吸光度进行逐步回归，建立6种指标成分含量的UV预测方程，另取8批周氏方提取液分别用HPLC和UV预测法测定其实际含量和预测含量，检验预测结果的可靠性。结果：6种指标成分UV预测方程的相关系数依次为0.935，0.963，0.911，0.956，0.926，0.890。8批验证提取液中各指标成分的实际含量与预测含量结果的相关系数分别为0.946 5，0.976 5，0.907 9，0.981 9，0.911 4，0.896 2；两组值间偏差小；经t检验，两组数据间没有显著性差异。结论：UV预测法结果可靠、准确，方法快速、方便，可用于周氏方生产过程中6种黄酮指标成分的在线检测。

Abstract:

Objective: To establish the rapid UV on-line detection for six flavonoids ingredi-ents of zhou's prescription. Method: Six flavonoids index ingredients contents (catechin, hydroxysafflor yellow A, hyperoside, isoquercitrin, quercitrin quercetin) in 39 batches of zhou's extracting solution were measured by HPLC. The UV spectra of the same samples' were scanned and take the UV absorbance value among 200 nm to 500 nm every 1 nm. The wavelength which were detected by the above method and the six flavonoids index ingre-dients contents which were determined by HPLC were chosen to stepwise regress analyze and establish the prediction equations of six flavonoids index ingredients.Other 8 batches of extracting solution of zhou's prescription were determined by HPLC and UV prediction to test the UV prediction method. Result: The correlation coefficients of equations of eac-h flavonoids index ingredients were:catechin, 0.935;hydroxysafflor yellow A:0.963;hyp-eroside:0.911;isoquercitrin:0.956;quercitrin:0.926;quercetin:0.890.The correlati-on coefficients of six flavonoids contents determined by HPLC and UV prediction were:catechin, 0.946 5;hydroxysafflor yellow A:0.976 5;hyperoside:0.907 9;isoquercitrin:0.981 9;quercitrin:0.911 4;quercetin:0.896 2.The deviation of six flavonoids index ingredi-ents contents measured by the two methods were low.T-test show that there was no significant difference between UV prediction and HPLC method. Conclusion: The result of UV prediction was reliable and accurate, the UV prediction method was rapide and convenient, so it can be used for on-line testing six flavonoids ingredients in producting of Zhou's prescription.