黑面神枝叶中表儿茶素的含量测定及水提工艺优化

Quantitative Determination and Orthogonal Test of Water Extraction Conditions for Epicatechin in Leaves of Breynia fruticosa

摘要:

目的：测定黑面神枝叶中表儿茶素含量并优化水提取工艺条件。方法：采用HPLC法测定黑面神枝叶中表儿茶素含量，并运用正交试验考察浸泡时间、加水量、提取时间和提取次数对表儿茶素含量的影响。结果：表儿茶素在0.006 6~0.264 0 g·L^-1线性关系良好，回归方程Y=59 283X+53.986，r=0.999 9，平均回收率达98.2%，RSD 1.03%。水提最佳工艺条件：药材加20倍量水浸泡45 min，80 ℃温浸提取2次，每次1 h。结论：HPLC法测定黑面神枝叶中表儿茶素含量操作简便，专属性强，经正交试验优化后含量有所提高，为黑面神的进一步开发奠定基础。

Abstract:

Objective: To establish the determination method and optimize the water extraction conditions for Epicatechin in leaves of Breynia fruticosa. Method: The content of Epicatechin in leaves of Breynia fruticosa was determined by using HPLC. Permeating time, ratio of solid to liquid, extraction time and extraction times for the affection of water extraction rate of Epicatechin in leaves of Breynia fruticosa were detected by the orthogonal test. Result: The linear range of Epicatechin was 0.006 6-0.264 0 g·L^-1, the standard curve of Epicatechin was as follows Y=59 283X+53.986, r=0.999 9, the average recovery of Epicatechin was 98.2% with RSD 1.03%. The optimal water extraction technics were as follows:ratio of solid to liquid at 1:20 (w/v) and permeating for 45 minutes, 80 ℃ extraction 2 times and 1 hour for each time. Conclusion: HPLC method for determination of Epicatechin in leaves of Breynia fruticosa is simple and selective.The content of Epicatechin was higher after orthogonal test, which can be used for the development of Breynia fruticosa.