鬼箭羽总黄酮含量测定方法的建立

Optimization of Determination for Total Flavonoids in Euonymus alatus

摘要:

目的：建立鬼箭羽总黄酮的含量测定方法并筛选其提取方法。方法：以芦丁为对照品，通过单因素试验优选显色方法和显色条件，建立总黄酮含量测定的方法学考察并优选其提取方法。结果：采用盐酸-镁粉反应比色法，芦丁在0.020~0.104 mg与吸光度呈良好线性关系（r=0.997），平均加样回收率99.424%（RSD 2.220%）。结论：盐酸-镁粉显色法具有精密度高、重复性好等优势，适用于鬼箭羽总黄酮的定量分析。鬼箭羽总黄酮的提取宜采用回流法。

Abstract:

Objective: To establish a method for determination of the content of total flavonoids in Euonymus alatus. Method: Selecting rutin as a reference substance, single factor tests were adopted to optimize colorimetry methods and conditions, determination of the content of total flavonoids was established and optimized its extraction method. Result: Hydrochloric acid-Mg reaction assay was adopted, rutin had a liner relationship with absorbance in the range of 0.021-0.104 mg (r=0.997), average recovery was 99.424% with RSD of 2.220%. Conclusion: HCl-Mg colorimetry method appeared to be an accurate, reliable and repeatable system for determination of total flavonoids in E. alatus.Total flavonoids in E.alatus should employ reflux extraction method.