南海红树林内生真菌ZH-4d代谢产物研究

Metabolites of Mangrove Endophytic Fungus ZH-4d from South China Sea

摘要:

目的：研究南海红树林内生真菌ZH-4d的代谢产物。方法：采用反复硅胶柱色谱法、Sephadex LH-20凝胶色谱法等进行分离纯化，并通过理化常数测定和光谱分析鉴定其化学结构。结果：从南海红树林内生真菌ZH-4d的培养液中分离得到6个代谢产物。经波谱解析，分别为citrinin（1），tenellic acid C（2），tenellic acid A（3），terretonin（4），echinulin（5），（E）-4，6-dimethoxy-3-[4-oxo-5-(prop-1-enyl)-4H-pyran-2-yl]-isobenzofuran-1（3H）-one（6）。初步药理活性显示化合物1对口腔癌细胞KB，KBv200的抗肿瘤活性分别为0.08，0.09 mmol·L^-1。结论：所有化合物均首次从南海红树林内生真菌ZH-4d中分离得到。

Abstract:

Objective: To study secondary metabolites of mangrove endophytic fungus No·ZH-4d from the south china sea. Method: The compounds were isolated by column chromatography and identified on the basia of physic-chemical constants and spectral analysis. Result: Six metabolites were obtained and elucidated as citrinin (1), tenellic acid C (2), tenellic acid A (3), terretonin (4), echinulin (5), (E) -4, 6-dimethoxy-3-[4-oxo-5-(prop-1-enyl)-4H-pyran-2-yl]-isobenzofuran-1 (3H)-one (6). In the preliminary bioassay, 1 show strong inhibittory activity on the KB, KBv200 with IC₅₀ values of 0.08, 0.09 mmol·L^-1, respectively. Conclusion: The six metabolites were obtained from endophytic fungus No·ZH-4d for the first time.