壮药“国虾薄”（绞股蓝）热处理产物中人参皂苷Rg3的分离与鉴定

Isolation and Identification of Ginsenoside Rg3 from Heat-processed Gocaekmbaw of Zhuang-medicine

摘要:

目的：分离、鉴定国虾薄（绞股蓝）热处理产物中皂苷类化合物。方法：国虾薄在温度125 ℃、压力0.24 MPa 的条件下，加热处理3 h，用80%乙醇加热回流提取3 h，通过大孔树脂HP-20、硅胶柱及反相柱色谱等分离手段对其热处理产物进行分离，并用离子阱飞行时间质谱（LCMS-IT-TOF）、核磁共振波谱（NMR）等数据鉴定其成分。结果：从壮药国虾薄热处理产物中首次分离获得20（S）-人参皂苷Rg₃和20（R）-人参皂苷Rg₃两种不同构型的稀有人参皂苷。结论：通过热处理的方法能从国虾薄中分离得到人参皂苷Rg₃，这为人参皂苷Rg₃的制备提供了一个新思路。

Abstract:

Objective: To isolate and identify saponins from Zhuang-medicine Gocaekmbaw (Gynostemma pentaphyllum) after heat-processing. Method: The leaves of gocaekmbaw were steam-heated at 125 ℃ and 0.24 MPa for 3 h, then extracted with 80% ethanol for 3 h. The extracts were isolated with chromatography using HP-20, silica gel and reversed ODS column. The constituents isolated from gocaekmbaw after heat-processing were identified with ¹H-NMR, ¹³C-NMR and LCMS-IT-TOF spectra. Result: Two different configurations of saponins were obtained from the heat-processed Gocaekmbaw. They were identified as 20 (R) -ginsenoside Rg₃ and 20 (S)-ginsenoside Rg₃ and there was no data reported before the present study. Conclusion: Through the method of heat-processing, ginsenoside Rg₃ can be gained from Gocaekmbaw. This may offer a new direction to the preparation of ginsenoside Rg₃.