药用植物三尖杉内生真菌的分离鉴定及抗菌活性

Isolation, Identification and Antimicrobial Activity of Endophytic Fungi from Medical Plant Cephalotaxus fortunei

摘要:

目的：研究药用植物三尖杉Cephalotaxus fortunei内生真菌的分离鉴定及抗菌活性。方法：用组织培养方法从野生三尖杉的根、茎、叶中分离内生真菌，采用滤纸片扩散法对分离得到的内生真菌的代谢产物做抑菌活性研究。结果：分别从三尖杉根、茎、叶中共分离得到30株内生真菌；抑菌活性研究显示，有20株内生真菌的代谢产物至少对1种靶标菌有抑制活性，占总分离菌株的66.7%，其中菌株CFEF013对所有靶标菌均有抑制作用；经形态学和分子生物学鉴定，对7株具有较强抑菌活性的菌株分属镰孢菌属Fusarium 4株，壳囊孢属Cytospora 1株，拟盘多毛孢属Pestalotiopsis 1株，毛霉菌属Mucor 1株。结论：药用植物三尖杉内生真菌种类具有一定的多样性，内生真菌代谢产物抑菌活性良好。

Abstract:

Objective: To isolate the endophytic fungi from Cephalotaxus fortunei and investigate their antimicrobial activities. Method: Endophytic fungi were isolated by tissue-culture method and antimicrobial activities of endophytic fungi were investigated by using filter paper method and the active strains were preliminarily identified by morphological and molecular method. Result: Thirty strains of endophytic fungi were isolated from the roots, stems and leaves of wild C. fortunei in Qinba mountain. 20 strains (66.67%) showed antimicrobial activity against one or more of the tested microbes, and the strain CFEF013 has strong inhibition activity to all target strains. By morphological and molecular identification, 7 strains with strong antimicrobial activity were classified into genera of Fusarium (4 strains), Cytospora (1 strains), Pestalotiopsis (1 strain) and Mucor (1 strain). Conclusion: There are multiple endophytic fungi in C. fortunei, and their metabolites have good antimicrobial activities.