山药多糖纳米粒的体外抗氧化活性

Nanoparticles in vitro Antioxidant Activity of Polysaccharides from Chinese Yam

摘要:

目的：湿磨法制备山药多糖纳米粒，星点设计优化最优处方。研究山药多糖纳米粒的体外抗氧化活性。方法：通过星点设计优化湿磨法制备的山药多糖纳米粒最优处方，SALD-2201激光粒度分布仪检测其平均粒径及粒度分布。通过化学实验法和试剂盒法研究过氧化氢、羟自由基、超氧阴离子自由基、DPPH的抗氧化能力，并测定其还原力与金属螯合力。结果：星点设计得最优工艺料液比1：24、球磨转速360 r·mim^-1、球磨时间36 h。SALD-2201激光粒度分布仪测定1.0%粒径在186 nm，80.0%粒径575 nm。经体外抗氧化实验证实，过氧化氢、DPPH、还原潜力与金属螯合力的自由基清除率随多糖浓度的增加而成增长趋势。其中山药多糖与其纳米粒对DPPH清除能力在0.5，4，6，8 g·L^-1质量浓度下均有极显著差异。超氧阴离子自由基与羟自由基的抗氧化能力亦与多糖浓度成正相关。结论：山药多糖及其纳米粒体外抗氧化活性良好，且山药多糖纳米粒的体外抗氧化活性优于山药多糖。

Abstract:

Objective: To prepare nanoparticles containing polysaccharides from Chinese Yam by using wet grinding, and opyomize the best prescription by central composite design. Method: To distribute test average particle size and particle size distribution by SALD-2201 laser particle size. Studies on 6 in vitro antioxidant indexes superoxide anion as free radical and hydrogen peroxide, hydroxyl radicals, DPPH, reduction potentials and metal chelating. Result: optimal preparation of Chinese Yam polysaccharides on nanoparticles technology as Liquid is 1:24, ball mill is 36 h, speed time of ball mill is 360 r·min^-1.The size of made from Nano-particle as 1% size is 186 nm, 80.0% size is 575 nm. Conclusion: The antioxidant in vitro experiments confirmed that the yams over nanoparticles in vitro antioxidant activity of polysaccharides from Chinese Yam polysaccharides.