新疆鼠尾草总酚酸的体外抗氧化活性

Antioxidant Activities of Total Salvianolic Acid from Salvia deserta in vitro

摘要:

目的：研究新疆鼠尾草总酚酸提取物和纯化物的体外抗氧化活性。方法：以40%乙醇提取和大孔吸附树脂纯化分别制备得到新疆鼠尾草总酚酸的提取物和纯化物，以维生素C（VC）为对照，通过体外化学模拟方法测定新疆鼠尾草总酚酸提取物和纯化物的还原能力、对·DPPH自由基、羟基自由基（·OH）和超氧自由基（O₂^-·）的清除能力，研究新疆鼠尾草总酚酸的抗氧化活性。结果：新疆鼠尾草总酚酸提取物和纯化物清除·DPPH自由基的作用50%清除浓度（IC₅₀，分别为0.029，0.039 g·L^-1）弱于VC（IC₅₀0.018 g·L^-1）；对羟基自由基（·OH）的清除作用（IC₅₀分别为0.212，0.165 g·L^-1）强于VC（IC₅₀0.356 g·L^-1）；对超氧自由基（O₂^-·）的清除能力（IC₅₀分别为3.735，1.913 g·L^-1）弱于VC（IC₅₀0.390 g·L^-1），其纯化物还具有一定的还原能力。结论：新疆鼠尾草总酚酸具有较强的抗氧化作用。

Abstract:

Objective: To determine the antioxidant activity of the total salvianolic acid from Salvia deserta Schang. Method: The extraction and purification of the salvianolic acid were prepared by using 40% ethanol extracted and macroporous adsorption resin purified respectively. The antioxidant activities of the extraction and purification of the total salvianolic acid were determined by·DPPH, ·OH, O₂^-· scavenging experiments and Fe³⁺ reduction capacity experiments in vitro.Results were compared with that of positive control vitamin C (VC). Result: The·DPPH scavenging assay of the extraction and purification of the total salvianolic acid (IC₅₀0.029, 0.039 g·L^-1) were weaker than VC (IC₅₀ 0.018 g·L^-1). The·OH scavenging assay (IC₅₀ 0.212, 0.165 g·L^-1) were higer than VC (IC₅₀ 0.356 g·L^-1).The O₂^-·scavenging assay (IC₅₀ 3.735, 1.913 g·L^-1) were weaker than VC (IC₅₀ 0.390 g·L^-1). The purificiation also has a certain reduction capacity. Conclusion: The total salvianolic acid from S. deserta possessed high antioxidant activity.