川椒方对小鼠变应性结膜炎肥大细胞脱颗粒的影响

Effect of Chuanjiao Decoction on Mast Cell Degranulation in Allergic Conjunctivitis with Mice

摘要:

目的：观察川椒方对小鼠变应性结膜炎肥大细胞脱颗粒的影响。方法：将68只Balb/c小鼠随机分为6组，即正常组8只，模型组（不给药）、阴性对照组（生理盐水ig）、川椒方水煎剂低、中、高剂量组（5.25，10.5，21.0 g·kg^-1，ig，每天1次，ig体积均为10 mL·kg^-1），每组各12只。用鸡卵清白蛋白免疫小鼠建立变应性结膜炎模型。ELISA检测小鼠血清中前列腺素D2（PGD2）、组胺（HIS）和肿瘤坏死因子（TNF-α）的表达水平，RT-PCR观察结膜组织中白介素-4（IL-4）和TNF-α的表达。结果：与正常组相比，模型组和阴性对照组结膜肥大细胞聚集，脱颗粒明显（P<0.01），血清中PGD2，HIS和TNF-α的表达升高（P<0.01），结膜组织中IL-4和TNF-α的表达升高（P<0.01）。与模型组比较，川椒方各组结膜组织中肥大细胞数量减少，脱颗粒率下降（P<0.01）；血清中PGD2，HIS和TNF-α的表达显著降低（P<0.01）；结膜组织中IL-4和TNF-α的表达明显降低（P<0.01）。结论：川椒方能够治疗变应性结膜炎，其作用机制可能是通过稳定肥大细胞，抑制其脱颗粒。

Abstract:

Objective: To observe the effects of Chuanjiao decoction (CJD) on mast cell (MC) degranulation in allergic conjunctivitis (AC) with mice. Method: Sixty-eight mice were randomlly divided into the normal group, the model group, the placebo group (12 mice), the low, medium, and high dose CJD groups. After induced allergic conjunctivtis by intraperitoneal injection ovalbumin, mice in the normal group and model group were not gastrogavage, mice in the placebo group were given 10 mL·kg^-1 normal saline by gastrogavage, once daily for 7 days. Mice in the low, medium, and high dose CJD groups were given with CJD 10 mL·kg^-1 at the dose of 5.25, 10.5, 21.0 g·kg^-1 by gastrogavage, once daily for 7 days. The conjunctiva tissue were dyed by immunohistochemisty staining, the number of total MC and degranulated MC were counted.The concentration of prostaglandin D2 (PGD2), histamine (HIS) and tumor necrosis factor-alpha (TNF-α) in mice serum were measured by ELISA. The mRNA expression of interleukin 4 (IL-4) and TNF-α in mice conjunctiva were detected using RT-PCR. Result: Compared with the normal group, the number of total MC and degranulated MC were increased in the model and placebo group (P<0.01) ;the expression of PGD2, HIS and TNF-α in mice serum significantly increased in the model and placebo group (P<0.01). The expression of IL-4 and TNF-α in mice conjunctiva significantly increased in the model and placebo group (P<0.01). Compared with the model and placebo group, the number of total MC and degranulated MC were decreased in the CJD groups (P<0.01); the expression of PGD2, HIS and TNF-α in mice serum significantly decreased in the CJD groups (P<0.01) ;the expression of IL-4 and TNF-α in mice conjunctiva significantly decreased in the CJD groups (P<0.01). There was no statistical difference among the CJD groups. Conclusion: The action mechanism of CJD in treating AC might be achieved through stabilized and inhibited degranulation of mast cells.