蒙药槟榔十三味丸对抑郁模型大鼠神经-内分泌-免疫功能的影响

Effects of Mongolian Pharmaceutical Betel Shisanwei Ingredients Pill on Nerve-endocrine-immune Function in Depressive Rats

摘要:

目的：探讨蒙药槟榔十三味丸对慢性应激抑郁模型大鼠神经-内分泌-免疫功能的影响及治疗抑郁症的机制。方法：将Wistar雄性大鼠，根据蔗糖水消耗量及体重随机分为正常对照组、模型组、氟西汀组（3.3 mg·kg^-1）、槟榔十三味丸低、中、高剂量组（0.25，0.5，1 g·kg^-1），每组10只。除正常对照组外，其余大鼠均采用慢性轻度不可预见性应激结合孤养方法制备抑郁模型，造模同时灌胃给药，每日1次，连续给药28 d。实验结束后，迅速断头取血，分离血清，采用酶联免疫吸附法（ELISA）检测促肾上腺皮质激素释放激素（CRH）、促肾上腺皮质激素（ACTH）、皮质酮（CORT）、三碘甲状腺原氨酸（T₃）、四碘甲状腺原氨酸（T₄）、促甲状腺激素（TSH）、白介素-2（IL-2）、白介素-6（IL-6）的含量，并取胸腺、脾脏，计算胸腺指数、脾脏指数。结果：与正常对照组比较，模型组大鼠血清CRH，ACTH，CORT，T₃，IL-6含量明显升高（P<0.01），TSH含量、脾脏指数明显降低（P<0.01）；与模型组比较，氟西汀组和槟榔十三味丸大鼠血清CRH，ACTH，CORT，T₃，IL-6含量明显降低（P<0.01或P<0.05）、TSH含量明显升高（P<0.05或P<0.01），槟榔十三味丸组大鼠脾脏指数明显升高（P<0.01），尤其槟榔十三味丸高剂量组作用显著。结论：槟榔十三味丸能调节抑郁模型大鼠下丘脑-垂体-肾上腺轴和下丘脑-垂体-甲状腺轴功能，以及免疫功能，这可能是该复方抗抑郁作用的机制之一。

Abstract:

Objective: To study the effects of Mongolian pharmaceutical Betel Shisanwei ingredients pill (BSIP) on function of nerve-endocrine-immune in chronic unpredictable stress depression rat and therapeutic mechanism of depression. Method: Sixty male Wistar rats were randomly divided into six groups according to the sugar consumption test and body weight (10 rats in each group) :Normal control group, model group, Fluoxetine group (3.3 mg·kg^-1) and high dose, medium dose and low dose group (0.25, 0.5, 1 g·kg^-1) of BSIP.Except the normal control, the other groups were treated with the chronic unpredictable mild stress stimulation combined with lonely raising for 28 days.10 mL·kg^-1 drugs was given to the rats, once daily, continuously for 28 days. After the experiment blood samples were collected and enzyme-linked immunosorbent (ELISA) was used to detect corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT), 3, 5, 3'-triiodothyronine (T₃), 3, 5, 3', 5'-tetraiodothyronine (T₄), thyroid stimulating hormone (TSH), interleukin-2 (IL-2), the content of interleukin-6 (IL-6), and take the thymus, spleen to calculate thymus index, and spleen index. Result: compared with normal control group, model group rats serum CRH, ACTH, CORT, T₃, IL-6 levels increased significantly (P<0.01), the TSH levels, spleen index significantly decreased (P<0.01) ;Compared with model group, in the fluoxetine group and BSIP group, the serum CRH, ACTH, CORT, T₃, IL-6 content significantly decreased (P<0.01 or P<0.01) ;the TSH levels increased significantly (P<0.05 or P<0.01) ;in the BSIP group, the spleen index increased significan tly (P<0.01), especially in the BSIP high dose group, it was significant. Conclusion: BSIP can adjust the depression model rats hypothalamus-pituitary-adrenal axis and hypothalamus-pituitary-thyroid axis function, and immune function, this could be one of mechanisms.