石菖蒲挥发油和水煎液的抗焦虑作用

Antianxiety Effects of Acorus tatarinowii Volatile Oil and Water Decoction

摘要:

目的：观察石菖蒲抗焦虑作用并探讨其作用机制。方法：将昆明种雄性小鼠随机分为8组（n=12），分别为模型对照组、阳性对照组、石菖蒲挥发油高、中、低剂量组（10，5，2.5 g·kg^-1·d^-1），石菖蒲水煎液高、中、低剂量组（20，15，10 g·kg^-1·d^-1），模型组ig生理盐水，阳性组ig地西泮（2.5 mg·kg^-1·d^-1），连续ig给药5 d，末次给药0.5 h后进行高架十字迷宫实验和明暗箱实验，观察石菖蒲对小鼠焦虑行为的干预作用。用酶联免疫法测定小鼠脑内γ-氨基丁酸（GABA），谷氨酸（Glu），五羟色胺（5-HT）的含量。结果：①石菖蒲挥发油高、低剂量组和水煎液中、低剂量组能明显增加小鼠在高架十字迷宫装置上的开臂滞留时间（OT%，P<0.05），能明显增加小鼠的开臂进入次数（OE%，P<0.05）。②石菖蒲挥发油剂量组和水煎液高剂量组能显著增加小鼠脑内GABA的含量（P<0.05）；石菖蒲挥发油中、低剂量组和水煎液3个剂量组能显著降低小鼠脑内Glu的含量（P<0.05）；石菖蒲挥发油3个剂量组和水煎液3个剂量组能显著降低小鼠脑内5-HT的含量（P<0.05）。结论：石菖蒲挥发油和水煎液在小鼠高架十字迷宫模型和明暗箱模型上均表现出抗焦虑作用，其作用效果与地西泮类似。作用机制可能与提高中枢GABA含量，降低Glu和5-HT含量有关。

Abstract:

Objective: To observe the antianxiety effects of Acorus tatarinowii and to explore its mechanism of action. Method: The Kunming male mice were randomly divided into 8 groups (n=12), the model group, control group, positive control group diazepam (DZP), volatile oil of A. tatarinowii (VO) high dose group, middle dose group and low dose group (10, 5, 2.5 g·kg^-1·d^-1), water decoction of A. tatarinowii (WD) high dose group, middle dose group and low dose group (20, 15, 10 g·kg^-1·d^-1), the model control group intragastric administration physiological saline, positive control group intragastric administration DZP (2.5 mg·kg^-1·d^-1), continuous intragastric administration five days, 0.5 h after the last intragastric administration, do the elevated plus maze test and light-dark transitions test, to observe the intervention effect of A. tatarinowii Schott on anxiety behavior in mice. Determine the content of r-aminobutyric acid (GABA), glutamic acid (Glu), serotonin (5-HT) in the mice brain by enzyme linked immunosorbent assay. Result: ①The VO high, low dose group and WD low dose group could significantly increase the mouse open arms in the elevated plus maze apparatuson the retention time of residence time than the open arm (OT) % (P<0.05) ;The VO high, middle dose group and WD middle, low dose group could obviously increase the number of open arm of mice in the elevated plus maze apparatus into the number of the number of open arm ratio (OE) % (P<0.05). ②The VO dose group and WD high dose group could significantly increase the content of GABA in brain tissue of mice (P<0.05).The VO middle, low group and WD dose group could significantly reduce the content of Glu in brain of mice (P<0.05). The VO dose group and WD dose group could significantly reduce the content of 5-HT in brain of mice (P<0.05). Conclusion: The VO dose group and WD dose group of A.tatarinowii in mice with elevated plus maze model and light-dark transitions test model showed anti anxiety effect, its effect is similar with diazepam. The mechanism may be related to the increasing GABA content, reducing the content of 5-HT and Glu.