DA-201C型大孔树脂对土鳖虫酶解液的除盐工艺考察

Inspection for Desalting Process of Eupolyphaga Enzymatic Hydrolysate with DA-201C Macroporous Resin

摘要:

目的: 考察DA-201C型大孔树脂对土鳖虫酶解液的脱盐纯化效果.方法: 采用DA-201C型树脂对土鳖虫酶解液进行脱盐试验,通过单因素试验考察上样液pH和质量浓度、洗脱剂种类和用量对土鳖虫小肽的影响.通过folin-酚法测定活性成分小肽含量,检测波长650 nm,利用电导率测定除盐率.结果: 最佳除盐工艺为上样液与树脂柱体积比1:1,上样液质量浓度10 mg ·L^-1,上样液pH 7.0,吸附流速1.5 BV ·h^-1,加水3 BV洗除杂,加75%乙醇3 BV洗脱.除盐率99.1%,小肽回收率86.05%.结论: 采用DA-201C型大孔树脂对土鳖虫酶解液进行纯化除盐处理的方法简便、易行.

Abstract:

Objective:To investigate desalination purification effect of Eupolyphaga enzymatic hydrolysate by DA-201C macroporous resin. Method: Desalination test of Eupolyphaga enzymatic hydrolysate was adopted by DA-201C macroporous resin, effects of pH and concentration of sample solution, type and amount of eluent on Eupolyphaga small peptides were investigated by single factor tests.Folin method was employed to determine the content of small peptides with detection wavelength at 650 nm. Result: Optimum desalination process was as follows:resin-sample solution of 1: 1, the concentration of sample solution for 10 mg · L^-1, pH of 7.0, adsorption rate of 1.5 BV · h^-1, washed impurity with 3 BV of water and eluted by 3 BV of 75% ethanol;desalination rate was 99.1% with small peptides recovery of 86.05%. Conclusion: This optimized desalination process is simple and feasible, it can achieve desired purpose of Eupolyphaga enzymatic hydrolysate.