星点设计-响应面法优选藤梨根总皂苷提取工艺

Optimization of Extraction Technology of Total Saponins from Actinidia arguta Roots by Central Composite Design-response Surface Methodology

摘要:

目的: 优选藤梨根总皂苷的提取工艺,为该药用部分的开发提供参考.方法: 以总皂苷提取量为指标,采用星点设计-响应面法考察提取时间、乙醇体积分数和料液比对藤梨根总皂苷提取工艺的影响.利用UV测定总皂苷含量,检测波长547 nm.结果: 最佳工艺条件为提取时间73 min,乙醇体积分数65%,料液比1:13;藤梨根总皂苷提取量1.67 mg ·g^-1,与预测值1.69 mg ·g^-1的偏差1.18%.结论: 该提取工艺稳定可行,适用于藤梨根总皂苷的提取.

Abstract:

Objective:To optimize extraction technology of total saponins from Actinidia arguta roots and provide a reference for development of this medicinal part. Method: With extraction time, ethanol concentration and ratio of solid to liquid as independent variables, extracting amount of total saponins as dependent variable, extraction technology of total saponins from Actinidia arguta roots was optimized by central composite design-response surface methodology.UV was employed to determine the content of total saponins with detection wavelength at 547 nm. Result: Optimum technological parameters were as follows:extraction time of 73 min, ethanol concentration of 65%, solid-liquid of 1: 13;extracting amount of total saponins was 1.67 mg · g^-1, which was coincided with the model predictive value of 1.69 mg · g^-1. Conclusion: This optimized extraction technology is simple, reliable and suitable for extracting total saponins from A. arguta roots.