正交试验优选九龙藤总黄酮纯化工艺

Orthogonal Design for Optimizing Purification Process of Total Flavonoids from Bauhinia championii

摘要:

目的: 优选D101型大孔树脂纯化九龙藤总黄酮的工艺条件,为该成分的工业制剂开发提供参考.方法: 以总黄酮纯度及回收率为评价指标,在单因素试验基础上,采用正交试验考察上样液质量浓度、洗脱剂浓度、洗脱速度及上样液pH对九龙藤总黄酮大孔树脂纯化工艺的影响.采用UV测定黄芩苷含量,检测波长278.5 nm.结果: 最佳纯化工艺为上样液质量浓度15.5 g ·L^-1,上样液pH 2~3,最大上样量16.85 mg ·g^-1,上样速度0.125 BV ·h^-1,径高比1:6,加60%乙醇4 BV以1 BV ·h^-1洗脱.总黄酮纯度48.95%,回收率96.08%.结论: 优选的纯化工艺稳定可行,有效提高了九龙藤总黄酮纯度及回收率,适用于九龙藤总黄酮的纯化.

Abstract:

Objective:To optimize purification process of total flavonoids from Bauhinia championii by D101 macroporous resin and provide a reference for preparations development of these components. Method: With purity and recovery of total flavonoids as indexes, based on single factor tests, orthogonal design was used to optimize purification process by taking eluent concentration, elution rate, pH and concentration of sample solution as factors.UV was employed to determine the content of baicalin with detection wavelength at 278.5 nm. Result: Optimal purification process was as following:the concentration of sample solution 15.5 g · L^-1, pH of sample solution 2-3, the maximum sample volume of 16.85 mg · g^-1, sampling rate of 0.125 BV · h^-1, diameter and height ratio of 1: 6, eluted by 4 BV of 60% ethanol with elution speed of 1 BV · h^-1.Under this process, purity and recovery were 48.95% and 96.08%, respectively. Conclusion: This optimized purification process is stable, feasible and suitable for purifying total flavonoids from B. championii, which can effectively improve its purity and recovery.