菟丝子的酶法提取工艺优选

Optimization of Enzymatic Extraction Technology of Cuscutae Semen

摘要:

目的: 优选菟丝子的酶法提取工艺,以提高菟丝子中金丝桃苷及总黄酮的提取率.方法: 以金丝桃苷提取量为指标,通过单因素试验考察酶种类、酶用量、酶解时间、酶解pH及温度对酶解效果的影响;以金丝桃苷与总黄酮提取量的综合评分为指标,采用正交试验考察酶用量、酶解pH及酶解温度对酶解工艺的影响.采用HPLC测定金丝桃苷含量,流动相乙腈-0.1%磷酸溶液(17:83),检测波长360 nm.结果: 选择纤维素酶,最佳提取工艺为酶用量2.0%,酶解时间2.0 h,酶解pH 4.0,酶解温度45 ℃;金丝桃苷、总黄酮提取量分别为1.408,26.966 mg ·g^-1.结论: 优选的酶法提取工艺稳定合理,适用于菟丝子的工业生产.

Abstract:

Objective:To optimize enzymatic extraction process of Cuscutae Semen, in order to increase extracting rates of hyperin and total flavonoids. Method: With the content of hyperin as index, effects of enzyme species, enzyme dosage, enzymolysis time, pH and temperature on enzymatic extraction process were investigated by single factor test.With composite score of extracting amounts of hyperin and total flavonoids as index, orthogonal test was used to optimize enzymatic extraction process with enzyme dosage, enzymolysis pH and temperature as factors.HPLC was employed to determine the content of hyperin with mobile phase of acetonitrile-0.1% phosphoric acid solution(17: 83) and detection wavelength at 360 nm. Result: Cellulase was selected, its optimum extraction technology was as follows:enzyme dosage 2.0%, enzymolysis time of 2 hours, enzymatic hydrolysis of pH 4.0, enzymolysis temperature at 45 ℃;extracting amounts of hyperin and total flavonoids were 1.408, 26.966 mg · g^-1, respectively. Conclusion: This optimized extracting process is stable, reasonable and applicable to industrial production of Cuscutae Semen.